If it smells of octanol (vaguely fruity, cloying odor) and possibly has a
little phase separation (octanol poorly mixes with water) then you have
significant hydrolysis.
Beyond the smell test, you can run a small sample out on a TLC with
n-Octanol as control lane. Pretty easy.
As to whether
Hi there,
I am using a stock solution of Octyl-Glucoside to crystallize my membrane
protein, which was made ~1 year before and kept at 4c . I wonder whether OG can
be hydrolysed under this condition and whether there is a way to test.
Many thanks,
Deliang
CCP4 bulletin board wrote on 02/25/2009 03:08:10
PM:
> On Wednesday 25 February 2009 08:20:14 Jayashankar wrote:
> > Dear Folks,
> >
> > The last novel proteins fold were from the yr 2007(pdb statistics),
> > From 2007 to till date no novel fold has been identified,
>
> If you reached this conclu
Must be even smaller than Daresbury then. They don't even have a
synchrotron!
Bart
James Holton wrote:
Paul Emsley wrote:
Here's an experiment:
Find a blindfold and put it on. Oh, but before you do that, take a
map of England and place it on a dartboard.
Now take 56066 darts and throw them
Paul Emsley wrote:
Here's an experiment:
Find a blindfold and put it on. Oh, but before you do that, take a
map of England and place it on a dartboard.
Now take 56066 darts and throw them at the map on the board.
Take off the blindfold and investigate where the darts hit. Did you
hit all the
Unfortunately the database contains huge bias towards:
a) proteins that are easy to make and crystallize
b) biologically interesting protein families
Artem
---
When the Weasel comes to give New Year's greetings to the Chickens no good
intentions are in his mind.
-Original Message-
From:
The protein wolves get it - and it's never heard from or seen again.
Artem
---
When the Weasel comes to give New Year's greetings to the Chickens no good
intentions are in his mind.
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Jacob
Keller
Sen
Some useful tips to try can be found at
http://www.embl-hamburg.de/services/protein/production/expression/optimising_exprlevels.html
I've had a recent case where an untagged protein (part of a complex)
was not expressed at all but expressed well when tagged at the
N-terminal with His6 or MBP. Also
What happens when a single protein strays from the fold?
Jacob
- Original Message -
From: "Edward A. Berry"
To:
Sent: Wednesday, February 25, 2009 2:48 PM
Subject: Re: [ccp4bb] protein folds
As an outsider to this field, it would seem another way to approach it
would be to ask, o
I agree with the accolades given by Raji to the CCPBB, but suggest a
difference: it is not given by "Nature," or any other polytheistic entity but
rather by the patience and sustained efforts of all the souls who resist the
urge to excoriate, which I would guess scales linearly to the number of
As an outsider to this field, it would seem another way to approach it
would be to ask, of the currently known folds, how many have only one
member (excluding evolutionarily related protein families)?
If a large number of folds have only been sampled once, it is likely
that there are others that
On Wednesday 25 February 2009 08:20:14 Jayashankar wrote:
> Dear Folks,
>
> The last novel proteins fold were from the yr 2007(pdb statistics),
> From 2007 to till date no novel fold has been identified,
If you reached this conclusion by looking at the PDB web site,
you should note that the site
Did you not know that the CCP4BB-- even when all else appears to be
melting down-- is Nature's sustained gift to humankind? Did you also
not know that astronauts, astronomers, astrologers, artists,
musicians, sportsmen, scientists, politicians, writers and everyone
else subscribes to the bo
Thanks to all who responded. Actually, this bulletin board is better for
help with molecular biology than the molecular biology bulletin board I am
subscribed to!
On Tue, Feb 24, 2009 at 7:47 PM, Stephen Weeks wrote:
> Mo,
> Just to add my 50 cents, I didn't see any mention of the use of fusion
But provided if i colud follow a pattern even blindfolded could come up with
some probable things.
Is it not ok to have principle of mathematical induction.
S.Jayashankar
Research Student
Institute for Biophysical Chemistry
Hannover Medical School
Germany.
On Wed, Feb 25, 2009 at 7:07 PM, Pau
Jayashankar wrote:
Dear Folks,
Dear gmail-user,
The last novel proteins fold were from the yr 2007(pdb statistics),
From 2007 to till date no novel fold has been identified, this mean
the present 1283 fold are the final or
should I wait, if so , with what criteria do I expect for a new
fol
Thank you Graeme.
I have installed ccp4 6.1.1 and ran the same job on SCALA succesfully.
J
Dr James Foadi PhD
Membrane Protein Laboratory
Diamond Light Source Ltd.
Diamond House
Harwell Science and Innovation Campus
Didcot
Oxfordshire
OX11 0DE
United Kingdom
office email: james.fo...@diamond.a
Dear Folks,
The last novel proteins fold were from the yr 2007(pdb statistics),
>From 2007 to till date no novel fold has been identified, this mean the
present 1283 fold are the final or
should I wait, if so , with what criteria do I expect for a new fold..or
what are the expectations ...
If we
I am currently looking for a PhD student to work on structure/function
studies of high-pressure adaptation by X-ray crystallography and
electron microscopy coupled to single particle analysis. The research
work will be carried out at the School of Biological Sciences (SBS)
and at the Centre
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