Hello Everyone
Since I'm convinced that ccp4bb is the repository of all knowledge
about all diffraction phenomena...
A colleague would like to do some fiber diffraction studies. I have
an RAxis - I know we can put the fiber in the beam and shoot it and
get some pattern on the detector.
Dear All,
Thank you very much for the reply and suggesions regarding my self rotation
function calculation. Really I appreciate who are all responded me regarding
my query. Now I got some idea about my structure in the assymetric unit.
Thanking you once again.
Sincerely yours,
Sampath
On Thu,
Dear Colleagues,
I'd like to share with you with a twinning puzzle that's been
troubling me for a while.
I have a ~2.2 A data merged in p212121 space group with unit cell:
41.8300 117.4490 134.4840 90. 90. 90.
Translational NCS was detected but structure solution was able to b
Sorry for this very prosaic question:
does anybody have a reference describing the mechanisms of reducing agents
(TCEP, DTT, BME, etc.), and in particular the effects of the reactions on
pH? I think I can draw a convincing reaction mechanism for myself, but I am
not sure theoretically why (or
I am trying to find a method to purify/separate phosphorylated protein
from unpshosphorylated proteins. I have two approach in my mind
* ion-exchange
* Fe columns, I tried this too but limited success.
Gadolinuim-charged IDA with a shallow gradient of imidazole is supposed to
separate ph
Hi there Satheesh,
In:
Activation Mechanism of the MAP Kinase ERK2 by Dual Phosphorylation .
Cell , Volume 90 , Issue 5 , Pages 859 - 869
B . Canagarajah , A . Khokhlatchev , M . Cobb , E . Goldsmith
(PDB # 2ERK)
the structure of phosphorylated ERK II was solved by co-expressing a
suitable, cons
Dear CCP4BB,
This is an off topic question for CCP4BB, I am posting on behalf of my friend:
Hi,
I am trying to find a method to purify/separate phosphorylated protein from
unpshosphorylated proteins. I have two approach in my mind
ion-exchange
Fe columns, I tried this too but limited
I am getting the following error in imosflm when
attempting to read an image file from an RAxis IV++ detector. I can
read RAxis IV image files just fine from the same facility. Is this a
header formatting issue? And is there a (relatively) simple way to fix
it? In MOSFLM (old GUI) attempting to
Use Coot
option 1)
rebuild your chain A and superimpose manually with Coot A to the other
chains, then write out a new merged PDB file
option 2)
use the Coot command which allows you to copy whatever you have
modeled in one chain to the other chains.
for more info check out the link:
http://
Dear all,
I am working with a relatively low res. crystal structure with
multiple protomers in the asymmetric unit related by NCS. I am
currently running rounds manual model building in Coot and refinement
using Refmac5. Could anybody suggest a shortcut for the following
procedure: I start
Hi Sampath,
Looks to me like you might have a hexamer with 32 symmetry in asymmetric
unit.
Ben
(I think if you were in the wrong space-group and the 3 fold was
crystallographic the peak height on the chi =120 section would be higher?)
"Sampath Natarajan" <[EMAIL PROTECTED]>
Sent by:
James,
1) It does not seem to have a 222 symmetry from the self-rotation
under P2/P21.
2) Besides what Eleanor suggested, there might be another possibility
that it is twinned in a way with P2/P21 cell of a'(=a), b'(~a, =b/2),
c'(c),
90, 90, 90. The uniqueaxis is along a' or b', but not c
James, when you tried P21, did you try all possibilities, i.e. P 1 21 1,
P 21 1 1, and P 1 1 21? Also, try the P2 versions thereof, and all eight
orthorhombic possibilities. I've had several crystals where the syst.
absences were misleading; pseudo-crystallographic NCS could account for
them. Dave
Hi,
>> 9. The selenomethionine dataset was solved using MIRAS in SHARP/autoSHARP.
>> The experimentally phased electron density yields contiguous tracts of
>> density in the right place, unbiased density indicates a good solution.
>> Model building was conducted in P212121, initially into the exp
Hi Sympath,
Did you try to change the default "Search radius"?
To my understanding MOLREP estimats the search radius somehow from the
volume of the asymmetric unit and not from the expected radius of the
monomer (you don't give the program the number of expected molecules).
The signal of the SR
Dear Colleague,
PHARE is a modular workshop on global phase retrieval to be held in Martina
Franca (Bari, Italy) on April 2009. The workshop is focused on
crystallographic methods for phase determination and refinement:
participants will be trained on the bases of the methods and on the latest
a
I guess my hunch would be that there is some sot of order-disorder
problem; either twinning or crystal dislocation.
Some ideas -
reindex the PG222 data set h/2,k,l so that a~=b and test that data for
twinning - you can just run truncate on the output Is and see the
moments and cumulative inten
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