Dear CCP4BB, This is an off topic question for CCP4BB, I am posting on behalf of my friend: Hi, I am trying to find a method to purify/separate phosphorylated protein from unpshosphorylated proteins. I have two approach in my mind
ion-exchange Fe columns, I tried this too but limited success. Has any one used any other method for this? It will be great if you can point some references in this regard. Another approach is to use commercially available kinase to phosphorylate the target protein – any comment on this? All suggestions are welcome. Thank you for your reply in advance.Thanks Satheesh Dr. P.Satheesh Kumar Department of Biochemistry and Biophysics Texas A&M University, 2128 TAMU College Station, TX - 77843-2128 USA. Ph. 1-979-862-7639 _________________________________________________________________ Searching for the best deals on travel? Visit MSN Travel. http://in.msn.com/coxandkings
