[ccp4bb] NCS and manual building

[Olga Boudker]

Dear all,
I am working with a relatively low res. crystal structure with 
multiple protomers in the asymmetric unit related by NCS.  I am 
currently running rounds manual model building in Coot and refinement 
using Refmac5.  Could anybody suggest a shortcut for the following 
procedure:  I start with an initial model containing all protomers; 
generate NCS operators for A to B, A to C et c.; rebuild protomer A; 
use the operators and the rebuilt A to generate all other protomers; 
and combine them all in a single pdb file.   Currently I do all that 
manually using lsqkab and text edit and it is really painful.  Is 
there an available routine to do this? For various reasons I would 
like to stick with Refmac5 rather then CNS, in which of course the 
symmetry would have been easier to implement.
Many thanks,
Olga
--