On Wednesday 12 November 2008 02:54:54 Ian Tickle wrote:
>
> All - I was just in a discussion about TLS and one thing that came out
> that I hadn't been aware of is that for the Biso restraints Refmac
> restrains the difference between the 'residual' Bs, i.e. with the TLS
> contributions subtracte
Dear Crystallographers,
A position is available for a postdoctoral fellow to join the
structure group in the Protein Engineering
Department at Genentech, Inc (Job code 100476). Active areas of
research include the structure and function
of intracellular signaling cascades involving ubiquitin
Hi all,
I'm trying to find a website where I can seach a nucleotide database with a
relatively short (say 12 bases) in-frame sequence (i.e; -GGA--CAG-etc), and
have the results in the same frame (so the resulting hits include regions
which code for the same amino acid sequence). I'm sure this can
Returning to the previous discussion, we've had luck crystallizing several
proteins with a His-tag. We've used both N-terminal and C-terminal his tags,
and have had success with both of them. In one of our structures, the his
tag actually formed an interface for crystal packing.
We have also tried
A PhD position in protein biochemistry is available in the group of
Prof. Utz Fischer at the Institute of Biochemistry, Biocentre of the
Julius-Maximilians-University, Wuerzburg/Germany.
The successful applicant will work on the Survival Motor Neuron (SMN)
complex, the key player during the
The original idea was to do with NCS restraints between B factors. The
idea is that TLS models the overall displacements which may be different
between NCS copies, while the residual Bs reflect local displacements
which ought to be similar.
Your argument applies restraints between bonded atoms, an
All - I was just in a discussion about TLS and one thing that came out
that I hadn't been aware of is that for the Biso restraints Refmac
restrains the difference between the 'residual' Bs, i.e. with the TLS
contributions subtracted, not the 'total' Bs. Now it seems to me that
this isn't quite c
disappointed users may be more likely to respond and their
testimony might reflect out-of-date versions,
Indeed! According to the emails I get, ARP/wARP never works!
Crystal Farm - (1 response, made/distributed by Bruker AXS)
Response was unfavourable. Noted reliability and service issues.
Summary on Crystal Imaging equipment
First, I would like to point out that the replies I received do not
amount to a representative survey that would accurately reflect the
performance of the instruments mentioned. Not all users of crystal
imaging system users read the BB, disappointed user
Dear All
just to let you know that the fee for the study weekend goes up from
£160 to £200 on 22 November.
Details, registration etc at
http://www.cse.scitech.ac.uk/events/CCP4_2009/
Charles Ballard
CCP4
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