[ccp4bb] 回复： RE: [ccp4bb] problem about Chains aw

Thank you very much. My operating system is Fedora5( I don't know whether this information is enough). "Stein, ND (Norman)" <[EMAIL PROTECTED]> 写道： Hi This is a problem that occasionally cropped up if there were large gaps in the alignment between target and model. It has been fixed

Re: [ccp4bb] coordinates of TCEP

Hi, if a compound is not in the hic-up data base of the USF (http://xray.bmc.uu.se/hicup/) I usually go to NCBI Pubchem database (TCEP e.g. at http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?cid=119411&loc=ec_rcs) , download the sdf file, feed it into a programm that can read it and put ou

[ccp4bb] coordinates of TCEP

Hi there, I suspect my reducing agent TCEP-HCl is bound to my protein in the crystal. I am trying to put a TCEP molecule into the bulky density, but could not find any available coordinates of TCEP. Do you know any where I can download the TCEP coordinates or any where I can build one starting from

Re: [ccp4bb] pH gradient in Mono Q

Hi Nadir, No I didn't and I am not concerning CHROMATOFOCUSING, and you are right, I have been thinking of using both pH and salt gradients to refine the separation. Thanks! Matt 2008/6/25 Nadir T. Mrabet <[EMAIL PROTECTED]>: > John, > > You can adjust your ionic strength not only with NaCl/KC

Re: [ccp4bb] pH gradient in Mono Q

John, You can adjust your ionic strength not only with NaCl/KCl/etc but also by increasing the concentration of the components in your buffer mixture. If you do it right, I can assure you can get a linear pH gradient. And, as mentioned earlier by Tom, you can use both pH and salt gradients to

Re: [ccp4bb] pH gradient in Mono Q

Thank you for all your advices. I usually employ salt gradient in MonoQ for my proteins, but I just wonder if we can use pH gradient in the same column and see if it can improve the separation. I know the condition that my protein binds to Mono Q, so I will try to titrate toward its pI to reduce

[ccp4bb] POST-DOCTORAL POSITION University of Nebraska, Lincoln, NE

Hi Folks, A post-doctoral position is available in the labs of Drs. Jay F. Storz and Hideaki Moriyama at the University of Nebraska. The NIH- funded research project involves an experimental investigation of molecular adaptation. Specifically, the goal is to identify mechanisms of hemoglob

[ccp4bb] PI Position @ SGC-Oxford: Integral Membrane Proteins

Hi, to spare us the need for head-hunters to identify ambitious researchers, check out below: We are seeking a Principal Investigator to lead the Integral Membrane Protein (IMP) group of the Structural Genomics Consortium (SGC), Oxford. The SGC is a charity organization that includes ~200 re

Re: [ccp4bb] Off-topic: Headhunters

Dear Bill, A person started his career as a Laboratory Technician and raised to a level of Associate Professor(and still dont know the next highest level) , this transitions in Sanskrit called as avatars (every state serve its purpose of its existence) , there is always good reasons for an indiv

Re: [ccp4bb] problem about Chainsaw

Hi This is a problem that occasionally cropped up if there were large gaps in the alignment between target and model. It has been fixed in the ccp4-6.0.99d test release. You can either download and install the test release, or if you let me know what operating system you are using, I will try