Re: [ccp4bb] Fwd: [ccp4bb] crystallisation robot

Actually, 3.5mins plate-to-plate for our 3-drop protocols (50:100, 75:75, 100:50ul) on our mosquito. (Not sure what protocol Artem is using, must have lots of tip changes.) Janet's reply reads slightly misleadingly: if you're concerned with plates/hour (which we are, since we have users show u

Re: [ccp4bb] Fwd: [ccp4bb] crystallisation robot

Dear Joe -- Does anyone have information about how long it takes to set up a 96- well tray for the crystallization robots available? Besides cost per tray and maintenance cost, another important feature we consider is the time for setting up a 96-well tray. It is an important factor sinc

Re: [ccp4bb] Fwd: [ccp4bb] crystallisation robot

About 2 - 2.5 minutes on Mosquito for single-drop protocol, scaled according to the number of drops per well (7 minutes for 3-drop trays). About 25 minutes on a Tecan-derived platform (numbers vary greatly depending on the particular configuration). Artem _ From: CCP4 bulletin boar

Re: [ccp4bb] Fwd: [ccp4bb] crystallisation robot

We have both a Phoenix and a Mosquito - for one-subwell 96 well plate both are under 3 minutes from the time you put the first droplet down until you have to seal the plate. The actual protocols take longer (particularly on the Phoenix) as you have to wash, transfer reservoir etc. We manage to fi

Re: [ccp4bb] Low resol structure

Dear Jim, 3.45 Angstrom is a perfectly normal resolution for a structure mediated by 'wobbly' detergent / lipid contacts. In this type of crystal the quality of the maps is the best indicator of intrinsic protein order. Send some density (in stereoview) to the journal as supplementary dat

Re: [ccp4bb] crystallisation robot

Hi Joe, We have a 32-head Honeybee robot which sets up a 96-well plate with a single drop per well in ~6 minutes and 3 drops per well at ~9 minutes. A 96-head phoenix or hummingbird-like system is likely going to be faster, but not by that much. Our robot has its own humidified cabinet and as

Re: [ccp4bb] Fwd: [ccp4bb] crystallisation robot

Depends on your robot, obviously. On Mon, Apr 14, 2008 at 10:32 PM, William Scott <[EMAIL PROTECTED]> wrote: > Does this include the customary grieving period? > > > > On Apr 14, 2008, at 2:10 PM, JOE CRYSTAL wrote: > > Hi, > > > > > > Does anyone have information about how long it takes to set

Re: [ccp4bb] Fwd: [ccp4bb] crystallisation robot

Does this include the customary grieving period? On Apr 14, 2008, at 2:10 PM, JOE CRYSTAL wrote: Hi, Does anyone have information about how long it takes to set up a 96- well tray for the crystallization robots available? Besides cost per tray and maintenance cost, another important feat

Re: [ccp4bb] Fwd: [ccp4bb] crystallisation robot

Hi, Does anyone have information about how long it takes to set up a 96-well tray for the crystallization robots available? Besides cost per tray and maintenance cost, another important feature we consider is the time for setting up a 96-well tray. It is an important factor since we are talking

Re: [ccp4bb] imosflm gets stuck - Mac OS 10.5.2 - solved.

Dear Harry, problem solved. You are a gentleman, sir! Thanks Ed -Original Message- From: Harry Powell [mailto:[EMAIL PROTECTED] Sent: Mon 4/14/2008 5:57 PM To: Thomas Edwards Cc: [EMAIL PROTECTED] Subject: Re: [ccp4bb] imosflm gets stuck - Mac OS 10.5.2 Hi Ed just a thought - was Mos

Re: [ccp4bb] anti-Validation conspiracy

No conspiracy here, but we have had a rough April thus far with MolProbity. We are having many "hung jobs" which we haven't addressed administratively (iow process killing). Right now, we're doing this manually and trying to determine reasons behind the "hangs". On Thu, Apr 10, 2008 at 10:17

[ccp4bb] SUMMARY: update of coot via fink

Problem was solved by fink selfupdate fink update-all Thanks to Chris Richardson, who suggested the above, and Andreas Förster, who suggested to be patient when launching updated coot the first time. Klaus -

Re: [ccp4bb] upgrade coot via fink on OS X

This is a gtk+2 phenomenon. Same happens with gimp-2. On Apr 14, 2008, at 8:36 AM, Andreas Förster wrote: Hey Klaus, after I installed coot 0.5 via fink for the first time, I encountered stalling during startup as well. However, this resolved itself within a minute. Startup resumed, and

Re: [ccp4bb] upgrade coot via fink on OS X

Hey Klaus, after I installed coot 0.5 via fink for the first time, I encountered stalling during startup as well. However, this resolved itself within a minute. Startup resumed, and the next time around, everything was back to normal. Were you patient enough during startup? Andreas Kl

[ccp4bb] heavy atom tutorial--thanks, and a summary

Thanks to all who responded to my query about cheap, easy, and foolproof systems for teaching students how to do MIR/SAD experiments. Below is a summary of responses. Pat - Summary follows - Jim Pflugrath: Lysozyme co-

[ccp4bb] imosflm gets stuck - Mac OS 10.5.2

Dear BB, Trying to install imosflm on my new Mac Pro running OS 10.5.2 imosflm will load the images, identify spots on the first frame, says it is trying to find spots on the last frame but then goes into spinning beachball mode and nothing happens. There is some data written out to mosflm.lp but

[ccp4bb] upgrade coot via fink on OS X

I had previously installed coot 0.3.2 using fink on OS X on MacBook Pro (under Tiger). Trying to update coot using fink proved more difficult. Initially I tried sudo apt-get install coot Coot would install but would not start due to a library version mismatch. Then it got messy. I followed in

Re: [ccp4bb] Need help for supperimpose 2 homologous molecules

If you just run SSM with the two files I think you will get the alignment you want? It seems to be able to sort out subunits from a whole model. Eleanor Paul Emsley wrote: > conancao wrote: > >> Dear all: >> >> I have problem to supperimpose 2 homologous proteins(only one subunit >> is homolog

[ccp4bb] Ninth Como meeting 29th September ­ 3rd October 2008

PRELIMINARY ANNOUNCEMENT Ninth International School on the Crystallography of Biological Macromolecules Como (Italy), 29th September ­ 3rd October 2008 www.crystallographyschool.org ORGANIZERS Keith Wilson (York University, UK) Derek Logan (Lund Universi