About 2 - 2.5 minutes on Mosquito for single-drop protocol, scaled according to the number of drops per well (7 minutes for 3-drop trays). About 25 minutes on a Tecan-derived platform (numbers vary greatly depending on the particular configuration).
Artem _____ From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of JOE CRYSTAL Sent: Monday, April 14, 2008 5:10 PM To: [email protected] Subject: Re: [ccp4bb] Fwd: [ccp4bb] crystallisation robot Hi, Does anyone have information about how long it takes to set up a 96-well tray for the crystallization robots available? Besides cost per tray and maintenance cost, another important feature we consider is the time for setting up a 96-well tray. It is an important factor since we are talking about sub-microliter drops. Best, Joe On Fri, Jan 18, 2008 at 12:28 PM, Lisa A Nagy <[EMAIL PROTECTED]> wrote: Al's Oil on the plates: What a nightmare!!!!!!! The oil creeps up the plate and over the sides. It dissolves adhesives. It makes me say bad words in multiple languages. Bigger drops + no oil = fewer bad words. Lisa -- Lisa A. Nagy, Ph.D. University of Alabama-Birmingham [EMAIL PROTECTED] -----Original Message----- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Patrick Shaw Stewart Sent: Friday, January 18, 2008 2:20 AM To: [email protected] Subject: [ccp4bb] Fwd: [ccp4bb] crystallisation robot One thing that people often overlook is that quite a lot of protein can be lost by denaturation on the surface of the drop. This is more significant for smaller drops. Two suggestions: (1) increase the proportion of protein in the - technical term - teeny drop to say two thirds and (2) cover the drops with oil eg Al's oils (silicone/paraffin). You still get vapor diffusion though the oil , and you'd like to slow up equilibration. of course (2) slows up the robotics a little, but both should be trivial to set up..
