Re: [ccp4bb] Glu+Arg solutions for solubility

Dear All, 1M is fine for either if you adjust the pH to 7 with the appropriate acid or base (NaOH or HCl). You can calculate an approximate amount using the HH equation. Also if you mix the two together, they are quite soluble. Mix Arginine and Glutamic acid, I mean. Obviously if you mix NaOH and

[ccp4bb] Refmac and mixed DNA base pairs

Hi All, I'm refining a structure of a protein DMA complex at 1.8A resolution. The spacegroup is P21 and the DNA is pseudopalindromic. It looks like I have electron density for mixed base pairs outside the palindromic region, but Refmac5 complains when I try to model it. ERROR: in chain E res

[ccp4bb] CNS problem: patch statement in generate.inp

Dear all, Sorry for a non-ccp4 question. I am trying to refine a structure of protein-RNA complex. The RNA have two 5-iodo-U in one chain. I used the following patch in generate.inp so that an I5 atom is added to the C5 atom of the Uracil. I add patch statement at the bottem of generate.inp {*

[ccp4bb] Axiom detector

Dear all, Has anybody out there experience with the Bruker Axiom detector? If yes, could you please give me some feedback? Many thanks in advance, Marie-Cécile Pelissier -- This message has been scanned for viruses and dangerous content by MailScanner, and is believed to be clean.

[ccp4bb] EMBO practical workshop, PEPC-6

The EMBL Hamburg Outstation is now accepting applications for the 6th biannual EMBO practical workshop on protein expression, purification and crystallisation (PEPC6) on September 1 – 9, 2008. This highly successful course features interactive lectures from internationally renowned researc

[ccp4bb] Postdoctoral Position at the University of Sussex

Postdoctoral Research Assistant in Protein Crystallography Ref: 131 This is a final reminder for this post. The closing date is the 8th March 2008. Applications are invited for a three year Postdoctoral position, funded by the MRC, in the laboratory of Dr. Darren Thompson. The successful c

[ccp4bb] BCA Spring Meeting

Hi all, Just a reminder that the cheap registration deadline for the BCA spring meeting is Monday March 3rd. Thanks to the coordinators of the BSG sessions, Arwen Pearson and Simon Phillips, the meeting is jam packed with sessions on: Membrane Proteins Neutrons in Biology Probing fast Biol

[ccp4bb] Post doctoral position in Helsinki

The Institute of Biotechnology is an independent research organisation within the University of Helsinki. The Research program for Structural Biology and Biophysics includes an X-ray Crystallography Unit equipped with an automated protein crystallisation facility and inhouse X-ray generator, in

Re: [ccp4bb] anomalous signal of Mn and Ca ions

On Feb 29, 2008, at 10:29, Johan Turkenburg wrote: Hi, You need to firstly check that you did the map calculation correctly, see comments below: Sun Tang wrote: Dear All, In my structures, I want to assign Mn or Ca ions for some densities. But when I did not have anomalous density in C

Re: [ccp4bb] anomalous signal of Mn and Ca ions

Hi, You need to firstly check that you did the map calculation correctly, see comments below: Sun Tang wrote: Dear All, In my structures, I want to assign Mn or Ca ions for some densities. But when I did not have anomalous density in CCP4i. I am not sure whether I was correct. The follow

Re: [ccp4bb] anomalous signal of Mn and Ca ions

As Ethan mentions, your best bet for an 100% positive ID would be to collect a dataset above and below the Mn edge and see the anomalous signal from the Mn atom disappear. If that is not an option, there is a great paper by George Sheldrick and friends regards using the Calcium Bond Valence Sum to

Re: [ccp4bb] Off topic: General rule for maximum flow rate for affinity column?

In general, I would wherever possible bind the protein in batch mode - 15-30 mins with occasional swirling. And then pour in a column, wash and elute. If washing and eluting is very slow, I would apply a little pressure that one time, but the next time make sure to choose a wider column. Anot