Re: [ccp4bb] Problem again with mosflm

Are your environment variables like $CINCL and $CCP4 defined? These usually get set up by sourcing the $CCP4/include/ccp4-setup.X file appropriate for your shell. michael nelson wrote: > Sorry folks, but I have been very frustrated to get > mosflm and imosflm to work. > I have get around with

[ccp4bb] Problem again with mosflm

Sorry folks, but I have been very frustrated to get mosflm and imosflm to work. I have get around with X11, but I got another problem, >> CCP4 library signal ccp4_general:Cannot open environ.def (Success) raised in ccp4fyp << ipmosflm_universal: Cannot open environ.def ipmosflm_

[ccp4bb] Visualization system for a Mosquito Robot

Hi all, sorry for the off topic post but we are in the market for a visualization system compatible with the Mosquito crystallization robot. We were just wondering what system, if any, do other crystallographers use as well as any experiences you may have had with it. Thanks for the input and t

Re: [ccp4bb] detergent concentration used for membrane protein purification

Hi Rongjin, During extraction from the membrane, typically 10xCMC (e.g. FC12) to 100xCMC (e.g. DDM) may be used, depending on target under study. During purification, it is recommended to bring this down to ~2xCMC. Theoretically, 1xCMC should be ok to keep the protein soluble. In practi

Re: [ccp4bb] detergent concentration used for membrane protein purification

Hi Rongjin, the concentration (and total amount) of detergent during purification depends a lot on what stage of purification you're at. For membrane extraction (1st step), people typically use anything from 1-5%. This depends on your budget, on the cmc of the detergent, and of course on the a

[ccp4bb] detergent concentration used for membrane protein purification

Dear All sorry for a non-ccp4 related question. what is the typical concentration of detergents used in membrane protein purification? Is it usually within 1-10 times of CMC? My collaborator was using 10% DDM in purifying a membrane protein. I suggested to reduce it and now he is working wit

[ccp4bb] Postdoc position at Genentech

Dear all, A position is available for a Postdoctoral researcher to join the crystallography group at Genentech, Inc. The group explores structural and functional aspects of proteins with scientific and/or therapeutic interest. The successful candidate will be involved in all aspects of crys

Re: [ccp4bb] Twinned crystals to single crystal

You can try e.g. - micro seeding - change MPD concentration (lower MPD especially with seeding) - scan the pH range - change protein concentration (lower protein conc. especially with seeding) - try different temperatures (4 degrees, 20 degrees, higher, if your protein supports it) - add anoth

[ccp4bb] SHELX reference

Since SHELX is frequently cited incorrectly, after some arm-twisting by the Acta Cryst. editors I have written a paper: "A short history of SHELX". Sheldrick, G.M. (2008). Acta Cryst. A64, 112-122 which may be safely cited whenever any program with a name beginning with "SHELX..." is used. I d

[ccp4bb] Twinned crystals to single crystal

Dear all, I have crystallized a protein in 63% of MPD at pH 3.8-4.6.The crystals are twinned but with sharp edges.I welcome all the suggestions to get single crystals.Also,I have needles of other mutant protein,the problem is during concentration,it gets precipitated even in the centricon.What shou