Dear All sorry for a non-ccp4 related question.
what is the typical concentration of detergents used in membrane protein purification? Is it usually within 1-10 times of CMC? My collaborator was using 10% DDM in purifying a membrane protein. I suggested to reduce it and now he is working with 2% DDM. He said lower than that the protein is not soluble. so another question is, can we purify the membrane protein first at high concentration of one detergent (say, 2% DDM), and then screen different detergents later to find a better one? Or it is better to screen the detergents first before purification? any suggestions will be appreciated. Have a nice holiday! Rongjin Guan
