I have small crystals of protein DNA complex. I am worried about the
possibility of presence of some
DNases in the drop (I use DNase in the lysis buffer). I have Tris and
MgCl2 in
the crystallization condition,
which makes me hesitant to use DEPC or EDTA/EGTA respectively. Are there any
other D
Hi all,
I have small crystals of protein DNA complex. I am worried about the
possibility of presence of some
DNases in the drop (I use DNase in the lysis buffer). I have Tris and MgCl2 in
the crystallization condition,
which makes me hesitant to use DEPC or EDTA/EGTA respectively. Are there an
Hi,
I suggest you check which version of MOLREP you used. Currently,
BALBES now actually use MOLREP in 'auto' mode for PST. The two should
be the same. The difference may be because BALBES uses the latest
version of MOLREP.
Fei
On 7/30/07, Eleanor Dodson <[EMAIL PROTECTED]> wrote:
> I think th
Dear all,
I tried to run DM for NCS averaging (DM module in CCP4i 6.0.2 installed
under Windows XP), but the running failed at different stages with various
error message as listed below. I was told it could be an installation
problem, but I don't know how to fix it. I am wondering if you have
I think the error is in BALBES - there is a peak I guess at 0.95 0 0.01
but it must be too close to the origin to be a translation vector from
one molecule to another.
There are reasons for such peaks - sometimes spurious large terms in the
data..
but they dont usually represent true molecula
Dear colleagues,
For a particular MR problem I am dealing with, 'analyse_mr' suggests
that there maybe a pseudo-translation vector as evidenced by the very
significant non-origin peaks in the native patterson: e.g
GRID 80 112 80
CELL 104.8290 151.2840 109.4910 90. 118.1310 90.
A
CNS format changes with the time of day and unfortunately f2mtz requires
that you give a correct fortran format stt.
This command script works from the GUI:
Note that
1) you must the "skip lines"
2) you need X entries to skip unwanted characters . These include INDE
(7x) FOBS= (6x) and also
