[Bioc-devel] Bioconductor 3.5 release: April 7 deadlines

2017-04-06 Thread Valerie Obenchain
Hi, Some recent activity has caused some (red) ripples across the builds and the El Capitan Mac builds are still unsettled. To accommodate, we're extending the deadline to pass R CMD build and check with no errors to next Friday (http://www.bioconductor.org/developers/release-schedule/). Than

Re: [Bioc-devel] Bioconductor 3.5 release: April 7 deadlines

2017-04-21 Thread Valerie Obenchain
tion to my package chimeraviz (so far it's in a separate branch and not commited to Bioconductor). Does the API freeze mean that I have to wait until the Bioconductor release before I commit it to the devel branch? On Fri, Apr 7, 2017 at 2:38 AM, Valerie Obenchain <mailto:voben...

[Bioc-devel] 3.5 branch created in svn

2017-04-24 Thread Valerie Obenchain
The BioC 3.5 branch is now ready. Remember, you always have access to 2 versions of your package in svn: the "release" and "devel" versions. Right now the "release" version of your package (which is not officially released yet but will be tomorrow if everything goes well) is in the 3.5 branch a

[Bioc-devel] Bioconductor 3.7 release candidate

2018-04-25 Thread Valerie Obenchain
Hi developers, Today is the deadline for updating NEWS files and marks the release candidate for Bioconductor 3.7. After today, pushes to master should be bug fixes only.   https://www.bioconductor.org/developers/release-schedule/ Thanks. Valerie [[alternative HTML version deleted]

Re: [Bioc-devel] GenomicFiles reducer and iterate argument

2014-06-17 Thread Valerie Obenchain
Hi Michael, Ryan, Yes, it would be ideal to have a single signature for both cases of 'iterate'. We went over the pros/cons again and at the end of the day decided to keep things as they are. No perfect solution here. These were the primary points: - Disadvantages of defining REDUCER with on

Re: [Bioc-devel] GenomicFiles reducer and iterate argument

2014-06-18 Thread Valerie Obenchain
Val's out today and I'm at least part of the problem so... On 06/17/2014 10:13 AM, Michael Lawrence wrote: On Tue, Jun 17, 2014 at 7:00 AM, Valerie Obenchain mailto:voben...@fhcrc.org>> wrote: Hi Michael, Ryan, Yes, it would be

[Bioc-devel] Bioconductor newsletter - July 2014

2014-07-01 Thread Valerie Obenchain
Now available at http://www.bioconductor.org/help/newsletters/ -- Valerie Obenchain Program in Computational Biology Fred Hutchinson Cancer Research Center 1100 Fairview Ave. N, Seattle, WA 98109 Email: voben...@fhcrc.org Phone: (206) 667-3158

Re: [Bioc-devel] writeVcf performance

2014-08-05 Thread Valerie Obenchain
Hi Michael, I'm interested in working on this. I'll discuss with Martin next week when we're both back in the office. Val On 08/05/14 07:46, Michael Lawrence wrote: Hi guys (Val, Martin, Herve): Anyone have an itch for optimization? The writeVcf function is currently a bottleneck in our

Re: [Bioc-devel] Additional summarizeOverlaps counting modes for ChIP-Seq

2014-08-06 Thread Valerie Obenchain
ut I think that either the preprocess.reads function should be required to only take one argument, or else the method of passing through additional arguments to it should be documented. -Ryan On Tue 05 Aug 2014 05:12:41 PM PDT, Ryan C. Thompson wrote: Hi Valerie, I got really busy around May and neve

Re: [Bioc-devel] Additional summarizeOverlaps counting modes for ChIP-Seq

2014-08-06 Thread Valerie Obenchain
all to summarizeOverlaps. These actually get passed straight to preprocess.reads. Perhaps the documentaion for "..." should be updated to reflect this? On Wed Aug 6 11:21:20 2014, Valerie Obenchain wrote: Hi Ryan, On 08/05/2014 05:47 PM, Ryan C. Thompson wrote: Hi again, I&#x

Re: [Bioc-devel] Parallel processing of reads in a single fastq file

2014-08-06 Thread Valerie Obenchain
d by a dedicated writer process?). Thanks, Jeff [[alternative HTML version deleted]] ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc-devel -- Valerie Obenchain Program in Computational Biology Fred H

Re: [Bioc-devel] Parallel processing of reads in a single fastq file

2014-08-19 Thread Valerie Obenchain
apply() is the absence of the trace function. Instead of having this hard coded we want to add a BPTRACE arg that allows tracing/debugging for any BiocParallel function. This should be added over the next week. Valerie On 08/06/2014 12:18 PM, Valerie Obenchain wrote: Hi Jeff, Thanks fo

Re: [Bioc-devel] writeVcf performance

2014-08-26 Thread Valerie Obenchain
5, 2014 at 2:33 PM, Valerie Obenchain mailto:voben...@fhcrc.org>> wrote: Hi Michael, I'm interested in working on this. I'll discuss with Martin next week when we're both back in the office. Val On 08/05/14 07:46, Michael Lawrence wrote:

Re: [Bioc-devel] writeVcf performance

2014-09-04 Thread Valerie Obenchain
tring manipulation in writeVcf. We either need to reduce it or reduce its overhead (i.e., the CHARSXP allocation). Gabe is doing more tests. On Tue, Aug 26, 2014 at 9:43 AM,

Re: [Bioc-devel] writeVcf performance

2014-09-08 Thread Valerie Obenchain
ows and the improvement over writeVcf() in release is ~ 90%. Valerie On 09/04/14 15:28, Valerie Obenchain wrote: Thanks Gabe. I should have something for you on Monday. Val On 09/04/2014 01:56 PM, Gabe Becker wrote: Val and Martin, Apologies for the delay. We realized that the Illumina platinum

Re: [Bioc-devel] writeVcf performance

2014-09-08 Thread Valerie Obenchain
fyi Martin found a bug with the treatment of list data (ie, Number = '.') in the header. Working on a fix ... Val On 09/08/2014 08:43 AM, Gabe Becker wrote: Val, That is great. I'll check this out and test it on our end. ~G On Mon, Sep 8, 2014 at 8:38 AM, Valerie Obencha

Re: [Bioc-devel] writeVcf performance

2014-09-09 Thread Valerie Obenchain
be rtracklayer). I'm working on the 'chunking' approach next. It looks like we can still gain from adding that. Valerie On 09/08/2014 12:00 PM, Valerie Obenchain wrote: fyi Martin found a bug with the treatment of list data (ie, Number = '.') in the header. Working on

Re: [Bioc-devel] writeVcf performance

2014-09-09 Thread Valerie Obenchain
Hi Herve, This unit test passes in VA 1.11.30 (the current version in svn). It was related to writeVcf(), not the IRanges/S4Vector stuff. My fault, not yours. Val On 09/09/2014 02:47 PM, Hervé Pagès wrote: Hi Val, On 09/09/2014 02:12 PM, Valerie Obenchain wrote: Writing 'list'

Re: [Bioc-devel] writeVcf performance

2014-09-17 Thread Valerie Obenchain
imenting with this. You can override default chunking with 'nchunk'. Examples on the man page. Valerie On 09/08/14 08:43, Gabe Becker wrote: Val, That is great. I'll check this out and test it on our end. ~G On Mon, Sep 8, 2014 at 8:38 AM, Valerie Obenchain mailto:voben...@fhcr

[Bioc-devel] thanks

2014-09-19 Thread Valerie Obenchain
Just a note to say thanks to those who worked on (1) the new biocViews search capabilities and (2) seqlevelsStyle<-. These are great improvements that have made tasks easier / faster time and time again. Yea! Val ___ Bioc-devel@r-project.org mailing

Re: [Bioc-devel] BiocParallel-devel error

2014-09-22 Thread Valerie Obenchain
Hi Thomas, Just wanted to let you know I saw this and am looking into it. Valerie On 09/20/2014 02:54 PM, Thomas Girke wrote: Hi Martin, Micheal and Vincent, If I run the following code, with the release version of BiocParallel then it works (took me some time to actually realize that), but w

Re: [Bioc-devel] BiocParallel-devel error

2014-09-23 Thread Valerie Obenchain
me know if you need more details, I can send another example off-line. Valerie On 09/22/2014 02:58 PM, Valerie Obenchain wrote: Hi Thomas, Just wanted to let you know I saw this and am looking into it. Valerie On 09/20/2014 02:54 PM, Thomas Girke wrote: Hi Martin, Micheal and Vincent, If I run

Re: [Bioc-devel] BiocParallel-devel error

2014-09-29 Thread Valerie Obenchain
Hi Michel, In BiocParallel 0.99.24 .convertToSimpleError() now checks for NULL and converts to NA_character_. I'm testing with BatchJobs 1.4, BiocParallel 0.99.24 and SLURM. I'm still not getting an informative error message: xx <- bplapply(1:2, FUN) SubmitJobs |++

Re: [Bioc-devel] GenomicFiles: chunking

2014-09-29 Thread Valerie Obenchain
Hi Kasper, The reduceBy* functions were intended to combine data across ranges or files. If we have 10 ranges and 3 files you can think of it as a 10 x 3 grid where we'll have 30 queries and therefore 30 pieces of information that can be combined across different dimensions. The request to '

Re: [Bioc-devel] writeVcf performance

2014-09-30 Thread Valerie Obenchain
rjson_0.2.14 [31] RSQLite_0.11.4 sendmailR_1.2-1 [33] splines_3.1.1 stringr_0.6.2 [35] survival_2.37-7tools_3.1.1 [37] TxDb.Hsapiens.BioMart.igis_2.3 XML_3.98-1.1 [39] xtable_1.7-4 zlibbioc_1.11.1 On Wed, Sep 17, 2014 at 2:08 PM, V

Re: [Bioc-devel] Cannot interact with a BigWig file on the web with rtracklayer (Windows specific)

2014-09-30 Thread Valerie Obenchain
Hi, Import and manipulation of BigWig files in rtracklayer make use of the Kent C library and are not supported on windows. This is documented at ?import.bw ?`BigWigFile-class` This problem isn't specific to an 'over the network' example. Trying to import() a local BigWig on Windows will al

[Bioc-devel] October Newsletter

2014-10-01 Thread Valerie Obenchain
The fourth quarter Newsletter is now available at http://www.bioconductor.org/help/newsletters/2014_October/ Topics include support for GRCh38, htslib and an introduction to the biocMultiAssay working group. Valerie ___ Bioc-devel@r-project.org mai

Re: [Bioc-devel] How to update annotation packages

2014-10-16 Thread Valerie Obenchain
Hi Robert, Annotation packages are generally updated before each release. You should have received an email from Marc (cc'd) last month asking if you had any updates. Updated packages are uploaded to our ftp server and Marc adds them to the repository. If you need to make a change I think Ma

Re: [Bioc-devel] VariantAnnotation::readVcf(fl, seqinfo(scanVcfHeader(fl)) problem

2014-10-23 Thread Valerie Obenchain
Hi Robert, Thanks for the bug report and reproducible example. Now fixed in release 1.12.2 and devel 1.13.4. I've also updated the docs to better explain how the Seqinfo objects are propagated / merged when supplied as 'genome'. Valerie On 10/23/2014 06:45 AM, Robert Castelo wrote: hi th

Re: [Bioc-devel] VariantAnnotation::readVcf(fl, seqinfo(scanVcfHeader(fl)) problem

2014-10-24 Thread Valerie Obenchain
of the Seqinfo object in the updated object? if not, would the solution be updating the 'genome' slot in the way i did it? thanks! robert. On 10/23/2014 11:14 PM, Valerie Obenchain wrote: Hi Robert, Thanks for the bug report and reproducible example. Now fixed in release 1.12.2 and d

Re: [Bioc-devel] GenomicFiles: chunking

2014-10-27 Thread Valerie Obenchain
s over chunks of GRanges. Within your MAP function: import(file, which = ranges, as = "Rle", format = "bw")[ranges] returns an RleList, and calling mean() on this gives a numeric vector of the mean of the coverage for each range. Then the only work needed is how to package t

Re: [Bioc-devel] GenomicFiles: chunking

2014-10-27 Thread Valerie Obenchain
Looks like the test code didn't make it through. Attaching again ... On 10/27/2014 11:35 AM, Valerie Obenchain wrote: Hi Kasper and Mike, I've added 2 new functions to GenomicFiles and deprecated the old classes. The vignette now has a graphic which (hopefully) clarifies the MA

Re: [Bioc-devel] GenomicFiles: chunking

2014-10-27 Thread Valerie Obenchain
r a subset of cells in the (ranges, files) matrix, and asking/estimating the amount of memory available for each worker. best, Mike On Mon, Oct 27, 2014 at 2:35 PM, Valerie Obenchain mailto:voben...@fhcrc.org>> wrote: Hi Kasper and Mike, I've added 2 new functions to Genomi

Re: [Bioc-devel] VariantAnnotation: Same locus, multiple samples

2014-12-08 Thread Valerie Obenchain
nges element. In other words, VCF is rectangular and VRanges is ragged, and there is no established way to encode the raggedness in the VCF. On Mon, Dec 8, 2014 at 11:27 AM, Valerie Obenchain mailto:voben...@fredhutch.org>> wrote: This could be fixed in the VRanges -> VCF coercion or in

Re: [Bioc-devel] VariantAnnotation: Harmonize default readVcf params

2014-12-08 Thread Valerie Obenchain
was just being too helpful, and the user ends up confused. So I agree with Julian that we should just drop VRangesScanVcfParam and have readVcfAsVRanges just be an alternative syntax to as(readVcf(), "VRanges"). Michael On Mon, Dec 8, 2014 at 10:48 AM, Valerie Obenchain mailto:voben...@f

Re: [Bioc-devel] VariantAnnotation: VCF to VRanges with multiple INFO values

2014-12-08 Thread Valerie Obenchain
Thanks to Michael and Julian for taking care of this. Fixes are in devel, >= 1.13.15. Valerie On 12/03/14 08:44, Michael Lawrence wrote: Looks like an issue when expand()ing the VCF. Maybe Val could take a look? On Wed, Dec 3, 2014 at 7:39 AM, Julian Gehring wrote: Hi, The conversion fro

Re: [Bioc-devel] VariantAnnotation: VCF to VRanges with multiple INFO values

2014-12-11 Thread Valerie Obenchain
Now in IRanges 2.0.1 and VariantAnnotation 1.12.7. Valerie On 12/11/2014 09:32 AM, Julian Gehring wrote: Can you backport the fixes to bioc-release which is also affected? Best Julian Valerie Obenchain (12/09/14 03:24): Thanks to Michael and Julian for taking care of this. Fixes are in

Re: [Bioc-devel] bamFlagAsBitMatrix error (?bug)

2014-12-14 Thread Valerie Obenchain
Hi Sean, Yes, we are aware of the problem, thanks. Hopefully this will be resolved for tomorrows builds ... we'll post back when it's fixed. Val On 12/14/2014 08:33 AM, Sean Davis wrote: Hi, Martin, Val, and Herve. This looks like a little problem with the bitnames in Rsamtools/GenomicAlign

Re: [Bioc-devel] bamFlagAsBitMatrix error (?bug)

2014-12-14 Thread Valerie Obenchain
gGraphs/inst/scripts/TSPC-utils.R SplicingGraphs/vignettes/SplicingGraphs.Rnw SplicingGraphs/man/assignReads.Rd SplicingGraphs/man/txpath-methods.Rd systemPipeR/R/utilities.R These packages should be clear (of this error) on tomorrow's builds. Thanks. Val On 12/14/2014 09:15 AM, Valerie

[Bioc-devel] January 2015 newsletter

2015-01-05 Thread Valerie Obenchain
Hi all, Happy 2015. The January 2015 Newsletter is now available: http://www.bioconductor.org/help/newsletters/2015_January/ Highlights include the (experimental) work being done with Docker containers, coordinate mapping, the algorithmic changes behind the overlaps operations and a review of

Re: [Bioc-devel] subtle error with VariantAnnotation::locateVariants()

2015-02-03 Thread Valerie Obenchain
Hi Robert, Thanks for reporting this. Now fixed in 1.13.27. I introduced this bug when switching the coordinate mapping over from mapCoords() to the new mapToTranscripts() family. The output from the new mapper is cleaner and allowed me to tidy some old code. In the process I realized there c

Re: [Bioc-devel] VariantAnnotation::isDelins() ??

2015-02-10 Thread Valerie Obenchain
Hi Robert, This sounds like a good addition. I'll put it on the TODO. If you need this immediately I'd be happy to accept a patch (with unit tests). Valerie On 02/10/2015 06:29 AM, Robert Castelo wrote: hi, in the VariantAnnotation package, the help of the functions for identifying varian

Re: [Bioc-devel] VariantAnnotation::isDelins() ??

2015-02-11 Thread Valerie Obenchain
s. cheers, robert. On 02/10/2015 07:37 PM, Valerie Obenchain wrote: Hi Robert, This sounds like a good addition. I'll put it on the TODO. If you need this immediately I'd be happy to accept a patch (with unit tests). Valerie On 02/10/2015 06:29 AM, Robert Castelo wrote: hi, in t

Re: [Bioc-devel] cryptic error in VariantAnnotation::locateVariants()

2015-02-16 Thread Valerie Obenchain
Hi, Thanks for the report. Now fixed in 1.13.31. This problem, like the last one you reported, was related to CDSID becoming an IntegerList. When region=AllVariants, results are combined and ordered by QUERYID, TXID, CDSID and GENEID. In your example ties were not broken by QUERYID or TXID an

Re: [Bioc-devel] GRanges to VRanges coercion

2015-02-23 Thread Valerie Obenchain
Hi Thomas, Michael, makeVRangesFromGRanges has been added to 1.13.34. The methods-VRanges-class.R file was already large so I added makeVRangesFromGRanges.R. A few notes: - '...' was replaced with 'keep.extra.columns' - row names are propagated automatically so I removed the explicit row.n

Re: [Bioc-devel] Changes to the SummarizedExperiment Class

2015-03-06 Thread Valerie Obenchain
Hi Mike, Our error - we didn't bump GenomicRanges when rowRanges was added. Hopefully 1.19.43 will propagate today and things will be sorted out. Val On 03/06/2015 07:40 AM, Michael Love wrote: hi all, just a practical issue: I have GenomicRanges version 1.19.42 on my computer which does n

Re: [Bioc-devel] requirement for named assays in SummarizedExperiment

2015-03-06 Thread Valerie Obenchain
Hi Aaron, Thanks for catching this. I favor enforcing names in 'assays'. Combining by position alone is too dangerous. I'm thinking of the VCF class where the genome information is stored in 'assays' and the fields are rarely in the same order. Looks like we also need a more informative erro

Re: [Bioc-devel] requirement for named assays in SummarizedExperiment

2015-03-11 Thread Valerie Obenchain
traint. Changes are in 1.19.46. Valerie On 03/06/2015 08:20 AM, Valerie Obenchain wrote: Hi Aaron, Thanks for catching this. I favor enforcing names in 'assays'. Combining by position alone is too dangerous. I'm thinking of the VCF class where the genome information is stored in

Re: [Bioc-devel] BiocParallel: changes to SnowParam(), should it import futile.logger?

2015-03-15 Thread Valerie Obenchain
Hi Leo, Thanks for reporting this. It was missed by the build system (and myself!) because the package was already installed. Should be fixed in 1.1.16. futile.logger (and others) were moved to Suggests to lighten the load of the NAMESPACE. Logging isn't technically turned on until the clust

Re: [Bioc-devel] ShortRead srdistance is broken

2015-03-16 Thread Valerie Obenchain
Hi Nico, Can you try again with BiocParallel 1.1.16? Likely related to this post, https://stat.ethz.ch/pipermail/bioc-devel/2015-March/007114.html Valerie On 03/16/2015 07:05 AM, Nicolas Delhomme wrote: Hej! Martin I guess that's for you :-) And I suppose it might only be temporary (due t

Re: [Bioc-devel] zero-width ranges representing insertions

2015-03-16 Thread Valerie Obenchain
On 03/16/2015 05:31 PM, Hervé Pagès wrote: On 03/16/2015 04:06 PM, Michael Lawrence wrote: On Mon, Mar 16, 2015 at 3:12 PM, Robert Castelo mailto:robert.cast...@upf.edu>> wrote: +1 IMO BioC could adopt the zero-width ranges representation for insertions, adapting readVcf(), writeVcf()

Re: [Bioc-devel] little tiny bug in CDSID annotations from predictCoding()

2015-03-17 Thread Valerie Obenchain
Hi Robert, Thanks for reporting the typo and bug. Now fixed in 1.13.41. Valerie On 03/17/2015 10:58 AM, Robert Castelo wrote: in my message below, the line that it says: head(loc_all$CDSID) it should say head(coding2$CDSID) cheers, robert. == hi,

Re: [Bioc-devel] little tiny bug in CDSID annotations from predictCoding()

2015-03-17 Thread Valerie Obenchain
D annotations that i described below the bug, between predictCoding() and locateVariants()? robert. Original message ---- From: Valerie Obenchain Date:17/03/2015 19:18 (GMT+01:00) To: bioc-devel@r-project.org Subject: Re: [Bioc-devel] little tiny bug in CDSID annotations from predic

Re: [Bioc-devel] zero-width ranges representing insertions

2015-03-19 Thread Valerie Obenchain
On 03/16/2015 05:31 PM, Hervé Pagès wrote: On 03/16/2015 04:06 PM, Michael Lawrence wrote: On Mon, Mar 16, 2015 at 3:12 PM, Robert Castelo mailto:robert.cast...@upf.edu>> wrote: +1 IMO BioC could adopt the zero-width ranges representation for insertions, adapting readVcf(), writeVcf()

[Bioc-devel] Bioconductor Newsletter - April 2015

2015-04-03 Thread Valerie Obenchain
Now available at http://bioconductor.org/help/newsletters/2015_April/ ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc-devel

[Bioc-devel] BioC 2015 Conference Posters

2015-04-24 Thread Valerie Obenchain
Poster registration is now open. Visit the web site http://www.bioconductor.org/help/course-materials/2015/BioC2015/ and registration page https://register.bioconductor.org/BioC2015/poster_submit.php for more information. Posters can be from any area of computational biology, medicine, c

Re: [Bioc-devel] rtracklayer::import( as = 'NumericList' ) doesn't work with more than 1 range

2015-05-20 Thread Valerie Obenchain
Hi Leo, Thanks for reporting the bug with import. The problem was in how the length of the output was computed. This has been fixed in both release (1.28.3) and devel (1.29.6). I'll let Michael answer the summary() question. Valerie On 05/19/2015 12:10 PM, Leonardo Collado Torres wrote: H

Re: [Bioc-devel] Redirect workers output to STDERR, how to do so with current BiocParallel::SnowParam()?

2015-05-22 Thread Valerie Obenchain
Hi, Thanks for reporting the bug. Now fixed in 1.13.4 (devel) and 1.2.2 (release). > bplapply(1:2, print, BPPARAM=SnowParam(outfile = NULL)) starting worker for localhost:11031 starting worker for localhost:11031 Type: EXEC [1] 1 Type: EXEC [1] 2 Type: DONE Type: DONE [[1]] [1] 1 [[2]] [1] 2

Re: [Bioc-devel] reproducible with mclapply?

2015-06-04 Thread Valerie Obenchain
I'll add a section to the BiocParallel docs. Valerie On 06/04/2015 07:55 AM, Kasper Daniel Hansen wrote: Yes, based on the documentation that particular random stream generator would work with mclapply. This is absolutely a subject which ought to be covered in the BiocParallel documentation.

Re: [Bioc-devel] strandless introns with VariantAnnotation::locateVariants()

2015-06-10 Thread Valerie Obenchain
Thanks for the report. Now fixed in release (1.14.2) and devel (1.3.25). Valerie On 06/09/2015 09:44 AM, Robert Castelo wrote: hi, currently, the annotation of variants in intronic regions by VariantAnnotation and the locateVariants() function does not assign strand to annotations in intron

Re: [Bioc-devel] strandless introns with VariantAnnotation::locateVariants()

2015-06-11 Thread Valerie Obenchain
enomicAlignments_1.4.1 BiocParallel_1.2.2 BSgenome_1.36.0 [5] tools_3.2.0 DBI_0.3.1 lambda.r_1.1.7 futile.logger_1.4.1 [9] rtracklayer_1.28.4 futile.options_1.0.0bitops_1.0-6 RCurl_1.95-4.6 [13] biomaRt_2.24.0 RSQLite_1.0.0 XML_3.98-

Re: [Bioc-devel] VRanges-class positive strandness and locateVariants() strandawareness

2015-06-11 Thread Valerie Obenchain
locateVariants(), predictCoding() and the family of mapToTranscripts() functions all return strand according to the annotation matched. The only time the strand of the output could possibly be different from the strand of the input 'query' is when 'ignore.strand = TRUE' (FALSE by default). I

Re: [Bioc-devel] VRanges-class positive strandness and locateVariants() strandawareness

2015-06-11 Thread Valerie Obenchain
sn't suggesting that LOCSTRAND be added to the locateVariants() output. Rather, it would be added to the VRanges during the merge. Michael On Thu, Jun 11, 2015 at 10:10 AM, Valerie Obenchain wrote: locateVariants(), predictCoding() and the family of mapToTranscripts() functions all retur

[Bioc-devel] Bioconductor Newsletter - July 2015

2015-07-01 Thread Valerie Obenchain
July newsletter available at http://www.bioconductor.org/help/newsletters/2015_July/ -- Computational Biology / Fred Hutchinson Cancer Research Center 1100 Fairview Ave. N, Seattle, WA 98109 Email: voben...@fredhutch.org Phone: (206) 667-3158 ___ Bio

Re: [Bioc-devel] Memory issues with BiocParallel::SnowParam()

2015-07-12 Thread Valerie Obenchain
Hi Leo, Thanks for the sample code I'll take a look. You're right, SnowParam has changed quite at bit - logging, error handling etc. The memory use you're seeing is a concern - thanks for reporting it. As an fyi, the log output for SnowParam and MulticoreParam now includes gc(), system.time

Re: [Bioc-devel] Memory issues with BiocParallel::SnowParam()

2015-07-12 Thread Valerie Obenchain
Vince, On 07/10/2015 04:12 AM, Vincent Carey wrote: I have had (potentially transient and environment-related) problems with bplapply in gQTLstats. Was the problem during build or check where a man page example or unit test could be isolated as the problem? I substituted the foreach abst

Re: [Bioc-devel] Redirect workers output to STDERR, how to do so with current BiocParallel::SnowParam()?

2015-07-14 Thread Valerie Obenchain
/vignettes/BiocParallel/inst/doc/Errors_Logs_And_Debugging.pdf there's a typo. It reads "SnowParma" instead of "SnowParam". Thanks, I'll fix that. Valerie On Fri, May 22, 2015 at 5:33 PM, Valerie Obenchain wrote: Hi, Thanks for reporting the bug. Now fixed in 1.13.4

Re: [Bioc-devel] Memory issues with BiocParallel::SnowParam()

2015-07-15 Thread Valerie Obenchain
as well as compare cluster environments. However, the gc() numbers from R 3.1.x (only available on the 4th replicate run) don't seem to support these thoughts. Or do you interpret these numbers differently? Best, Leo On Sun, Jul 12, 2015 at 11:00 AM, Valerie Obenchain wrote: Hi Leo,

Re: [Bioc-devel] Memory issues with BiocParallel::SnowParam()

2015-07-15 Thread Valerie Obenchain
Correction below. On 07/15/2015 04:14 PM, Valerie Obenchain wrote: Hi, BiocParallel in release and devel are quite similar so I'd like to narrow the focus to "before the changes to SnowParam" and after. This means BiocParallel 1.0.3 (R 3.1) vs BiocParallel 1.3.34 (R 3.2.1) which

Re: [Bioc-devel] VariantAnnotation: verbose output of readVcf

2015-07-16 Thread Valerie Obenchain
Hi Julian, Yes, the behavior is intentional though I hadn't thought of the annoying chatter in the case of chunking. Sorry about that. The current readVcf() reads/parses fields according to header multiplicity and type; fields without headers are skipped. It is on the TODO to be more liberal

Re: [Bioc-devel] testing class and length of function args

2015-07-22 Thread Valerie Obenchain
There is a collection in S4Vectors that test atomic types and return a logical. isSingleInteger isSingleNumber isSingleNumberOrNA isSingleString isSingleStringOrNA isTRUEorFALSE > isSingleNumber(1:5) [1] FALSE > isSingleNumber(NA) [1] FALSE Val On 07/22/2015 04:22 PM, Jim Hester wrote: No

Re: [Bioc-devel] VariantAnnotation: filterVcf with chunks duplicates header

2015-08-07 Thread Valerie Obenchain
Thanks for the report and reproducible example. Now fixed in 1.14.8 and 1.15.22. Valerie On 08/07/2015 07:25 AM, Julian Gehring wrote: Hi, When I use the 'filterVcf' function to process a VCF file in chunks, the output file contains as many copies of the header as there are chunks. Consider

Re: [Bioc-devel] small request on graph::graphBAM()

2013-02-07 Thread Valerie Obenchain
Hi Robert, I will look into this next week. Thanks for providing details and examples in the request. Valerie On 02/06/2013 04:10 PM, Robert Castelo wrote: dear maintainers of the graph package, i have a small request for the constructor function graphBAM() from the graph package. the curr

Re: [Bioc-devel] small request on graph::graphBAM()

2013-02-19 Thread Valerie Obenchain
ode(), which calls graphBAM(), was returning unsorted node names. To remain consistent with previous behavior I've sorted these results. Currently addNode() has no way to specify a full list of node names. Do you have an opinion on addNode() behavior? OK as is? Valerie On 02/07/20

Re: [Bioc-devel] VariantAnnotation: 'readVcf' returns incomplete 'CSQ' entry

2013-02-26 Thread Valerie Obenchain
Hi Julian, Thanks for the bug report. I agree this situation should be handled. It might be tomorrow before I get to this - I'll post back when it is done. Valerie On 02/26/2013 10:23 AM, Julian Gehring wrote: Hi Richard, That is true that is not according to the specs. However, the 'ensem

Re: [Bioc-devel] VariantAnnotation: 'readVcf' returns incomplete 'CSQ' entry

2013-02-27 Thread Valerie Obenchain
Julian, I'm trying to produce a vcf file with these premature endings for testing but am having no luck. Can you send me a small example of a vcf with the premature ends? Or maybe there is a combination of VEP flags that often result in this case? Thanks, Valerie On 02/26/13 09:50, Julian

Re: [Bioc-devel] ensemblVEP: 'fork' argument for 'VEPParam' should be integer

2013-02-27 Thread Valerie Obenchain
Thanks for catching this. A fix is checked in to 0.99.13. It's great to have a tester for this package. Let me know if you have suggestions/feedback for the user interface, how the param options are specified etc. Valerie On 02/27/13 07:25, Julian Gehring wrote: Hi, 'VEPParam' allows to s

Re: [Bioc-devel] VariantAnnotation: 'readVcf' returns incomplete 'CSQ' entry

2013-02-28 Thread Valerie Obenchain
minimal VCF file and code to reproduce this via e-mail. If you need more, please let me know. Best wishes Julian On 02/27/2013 08:53 PM, Valerie Obenchain wrote: Julian, I'm trying to produce a vcf file with these premature endings for testing but am having no luck. Can you send me a small

Re: [Bioc-devel] VariantAnnotation bug when loading multiple regions from vcf

2013-03-05 Thread Valerie Obenchain
Hi Moritz, Thanks for the bug report and suggested fix. The patch has been applied to 1.4.10 in release and 1.5.42 in devel. Valerie On 03/05/2013 07:05 AM, Moritz Gerstung wrote: Hi, I discovered the following problem in the geno() elements of the VCF object when I load multiple regions

Re: [Bioc-devel] contradictions in the return value of predictCoding() from VariantAnnotation

2013-03-09 Thread Valerie Obenchain
Hi Michael, Thanks for reporting the bug and sending a reproducible example. Sorry it took a few days to get to this. A fix has been checked into versions 1.4.12 (release) and 1.5.44 (devel). Valerie On 03/07/13 05:43, Michael Stadler wrote: Here is the second attachment ("variants.vcf") t

[Bioc-devel] Fwd: Fwd: [biocore] show for GRanges

2013-03-27 Thread Valerie Obenchain
Hi Vince, Martin forwarded your message about allowing more control over the show method. There are now 2 global options that control the number of lines displayed in the head and tail: showHeadLines showTailLines When set, these options affect GenomicRanges, GappedAlignments, Ranges and XS

Re: [Bioc-devel] Fwd: [BioC] Problem reading VCF file using readVcf from package VariantAnnotation

2013-04-25 Thread Valerie Obenchain
Hi Vince, Kasper, cc'ing Herve and Marc. I think we have a couple of things going on so I wanted to clarify. The 'genome' argument to readVcf() is assigned to the GRanges in rowData with the "genome<-" setter. This is where .normargGenome() gets called. setReplaceMethod("genome", "Seqinfo",

Re: [Bioc-devel] IntervalTree and Genomic Ranges

2013-05-29 Thread Valerie Obenchain
Hi Hector, Michael, This sounds great. Bringing these into svn is fine with us. Michael, do you want to merge these in? Val On 05/24/2013 07:30 AM, Hector Corrada Bravo wrote: Thanks Michael, It has made significant difference for our visualization project. I would like to merge this into s

Re: [Bioc-devel] deprecation of keepSeqlevels and renameSeqlevels

2013-05-29 Thread Valerie Obenchain
Hi all, keepSeqlevels(), dropSeqlevels(), renameSeqlevels() and the new restoreSeqlevels() are in GenomicRanges 1.13.16. Major changes: - These are high-level functions that wrap seqlevels(), not generics with methods. - All except restoreSeqlevels accept a named or unnamed character vecto

Re: [Bioc-devel] VcfFile and VcfFileList

2013-07-17 Thread Valerie Obenchain
Hi Robert, Have you seen TabixFile and TabixFileList? Both scanVcf() and readVcf() have methods for TabixFile. > showMethods("readVcf") Function: readVcf (package VariantAnnotation) file="character", genome="character", param="missing" file="character", genome="character", param="ScanVcfParam"

Re: [Bioc-devel] the character to collapse the geneNames when using the disjointExons function with aggregateGenes=TRUE

2013-07-31 Thread Valerie Obenchain
Hi Nico, (Adding Mike and Alejandro.) Because disjointExons() came from DEXSeq I wanted to preserve the behavior for backwards compatibility and familiarity to DEXSeq users. There are a couple of changes I'd like to make so disjointExons() is consistent with the other extractors in GenomicFea

Re: [Bioc-devel] the character to collapse the geneNames when using the disjointExons function with aggregateGenes=TRUE

2013-07-31 Thread Valerie Obenchain
Mike, Alejandro, I also wonder about getting rid of the 'exonID' metadata column. This is redundant with 'exonic_part_number'. Do you have a preference for keeping one or the other? Valerie On 07/31/2013 10:04 AM, Valerie Obenchain wrote: Hi Nico, (Adding Mike and

Re: [Bioc-devel] the character to collapse the geneNames when using the disjointExons function with aggregateGenes=TRUE

2013-08-01 Thread Valerie Obenchain
DEXSeq to those changes. Best regards, Alejandro Reyes Mike, Alejandro, I also wonder about getting rid of the 'exonID' metadata column. This is redundant with 'exonic_part_number'. Do you have a preference for keeping one or the other? Valerie On 07/31/2013 10:04 AM, Val

Re: [Bioc-devel] [devteam-bioc] Very slow when operate GRangesList

2013-08-23 Thread Valerie Obenchain
Hi Michael, Martin and I have been discussing this. In addition to the fix you suggest, what do you think of changing the default to compressed=TRUE for the RleList constructor? Rle is the only one of the AtomicLists with default FALSE. Was there a reason for this when it was first implemented

Re: [Bioc-devel] [devteam-bioc] Very slow when operate GRangesList

2013-08-27 Thread Valerie Obenchain
lerie On 08/23/2013 11:17 AM, Michael Lawrence wrote: On Fri, Aug 23, 2013 at 8:41 AM, Valerie Obenchain mailto:voben...@fhcrc.org>> wrote: Hi Michael, Martin and I have been discussing this. In addition to the fix you suggest, what do you think of changing the default to

Re: [Bioc-devel] expand,VCF and the AD geno field

2013-09-05 Thread Valerie Obenchain
Hi, On 09/04/2013 04:23 PM, Michael Lawrence wrote: Hi, Val, I just fixed expand,VCF to support multiple 'A' columns in info. Looks like expand,DataFrame does a cartesian product of list columns, which is not what we want in that case. Thanks. Ran up against another hurdle though: the conv

Re: [Bioc-devel] error in subsetting "ShortReadQ" object

2013-09-12 Thread Valerie Obenchain
The 'GroupedIRanges' error is related to some cleanup Herve is doing on the subsetting code in IRanges/XVector. The error should be gone with the most recent devel versions (IRanges 1.19.33, XVector 0.1.2, GenomicRanges 1.13.42). These may take a day to propagate through biocLite(). Valerie

Re: [Bioc-devel] Subsetting GRangesList with mcols on the mcols: Error in normalizeSingleBracketSubscript

2013-09-18 Thread Valerie Obenchain
Hi Michael, This looks like an artifact from reorganizing the subsetting code in IRanges/XVector. The operation works for me with the most recent versions from devel. I'm assuming it now works for you? Valerie On 09/13/2013 02:33 AM, Michael Love wrote: hi, I came across an error in trying

Re: [Bioc-devel] expand,VCF and the AD geno field

2013-09-24 Thread Valerie Obenchain
Michael, This is implemented in 1.7.48. AD is expanded as a list matrix where each list element holds a REF,ALT pair. Val On 09/05/2013 07:36 AM, Valerie Obenchain wrote: Hi, On 09/04/2013 04:23 PM, Michael Lawrence wrote: Hi, Val, I just fixed expand,VCF to support multiple 'A

Re: [Bioc-devel] VariantAnnotation writeVcf problem

2013-11-20 Thread Valerie Obenchain
Hi Jennifer, I've cc'd your message to the Bioconductor mailing list. We have two lists, one for general questions and the other for bug reports/feature requests. Please post future questions to one of these lists instead of sending them to a single person. The lists reach a wider audience and

Re: [Bioc-devel] [VariantAnnotation]

2013-11-20 Thread Valerie Obenchain
-’ for signature ‘"VCFHeader", "SimpleDataFrameList"’ Do you think that you will also implement this replacement method ? Thank you very much for your reactivity. Best regards. Delphine. Le 05/11/13 01:44, Valerie Obenchain a écrit : Hi Delphine, In 1.9.5 (devel br

Re: [Bioc-devel] [VariantAnnotation]

2013-11-20 Thread Valerie Obenchain
Correction below. On 11/20/2013 09:59 AM, Valerie Obenchain wrote: Hi Delphine, I've cc'd your message to the Bioconductor mailing list. We have two lists, one for general questions and the other for bug reports/feature requests. Please post future questions to one of these lists

Re: [Bioc-devel] Question for VariantAnnotation package for type G field

2013-11-20 Thread Valerie Obenchain
Hi Katsuhito, I've cc'd your message to the Bioconductor mailing list. We have two lists, one for general questions and the other for bug reports/feature requests. Please post future questions to one of these lists instead of sending them to a single person. The lists reach a wider audience an

Re: [Bioc-devel] IRanges::endoapply looks for FUN in the wrong places?

2013-11-21 Thread Valerie Obenchain
Sorry. This was likely my error when I created the replacement methods. I see you've fixed it in svn, Martin, thanks. Val On 11/20/2013 07:00 PM, Michael Lawrence wrote: Right, match.fun() needs to be within the initial endoapply, not later. Isn't the convention though to use `obeyQname<-` in

Re: [Bioc-devel] asVCF error coming from normalizeSingleBracketSubscript

2013-11-25 Thread Valerie Obenchain
Hi Stephanie, The error is thrown from SeqArray:::.info at line 216 in the file and is related to the handing of NA values. x[x == ""] <- NA Output from the == comparison can contain NAs and therefore can't be used (consistently) in subsetting operations. 'x' is a NumericList. Browse[2]

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