Re: [Bioc-devel] Question about external algorithms to Bioconductor package

2017-11-26 Thread Ioannis Vardaxis
.org>> Cc: "A.E.S." mailto:adrian.salat...@conicet.gov.ar>>, Ryan Thompson mailto:r...@thompsonclan.org>>, "bioc-devel@r-project.org<mailto:bioc-devel@r-project.org>" mailto:bioc-devel@r-project.org>> Subject: Re: [Bioc-devel] Question about ext

Re: [Bioc-devel] Question about external algorithms to Bioconductor package

2017-11-26 Thread Wei Shi
Date: Sunday, November 26, 2017 at 9:30 PM To: Martin Morgan Cc: "A.E.S." , Ryan Thompson , "bioc-devel@r-project.org" , Wei Shi Subject: Re: [Bioc-devel] Question about external algorithms to Bioconductor package I used Rbowtie and the mapping was done in 7 minutes, the res

Re: [Bioc-devel] Question about external algorithms to Bioconductor package

2017-11-26 Thread Ioannis Vardaxis
I used Rbowtie and the mapping was done in 7 minutes, the results where fine too. Rsubread had been running for 2 days so I had to stop it. But anyway I can use Rbowtie which is nice :) Ioannis Vardaxis Stipendiat NTNU Sendt fra min iPhone 26. nov. 2017 kl. 04:14 skrev Martin Morgan mailto:mar

Re: [Bioc-devel] Question about external algorithms to Bioconductor package

2017-11-26 Thread Wei Shi
.; Ryan Thompson Cc: bioc-devel@r-project.org; Wei Shi Subject: Re: [Bioc-devel] Question about external algorithms to Bioconductor package I think that generally Rsubread is 'fast' so you might make sure that there are not obvious problems, e.g., aligning reads to the wrong reference;

Re: [Bioc-devel] Question about external algorithms to Bioconductor package

2017-11-25 Thread Dario Strbenac
Good day, > I have DNA data. Is there any other solution rather than Rsubread which is > extremely slow? How much of it do you have? If it's a large size, such as whole genome sequencing, then it would take longer than an RNA-seq experiment regardless of the algorithm you use. I have used Rsub

Re: [Bioc-devel] Question about external algorithms to Bioconductor package

2017-11-25 Thread Martin Morgan
I think that generally Rsubread is 'fast' so you might make sure that there are not obvious problems, e.g., aligning reads to the wrong reference; maybe Wei Shi will chime in. Martin On 11/24/2017 09:57 AM, Ioannis Vardaxis wrote: Hi, I tried the Rsubread package you suggested and the mappin

Re: [Bioc-devel] Question about external algorithms to Bioconductor package

2017-11-24 Thread Martin Morgan
On 11/24/2017 01:25 PM, Ioannis Vardaxis wrote: Hei, Both kalliston and salmon er for RNA data, I have DNA data. Is there any other solution rather than Rsubread which is extremely slow? I am making an algorithm where one of its steps should be to map the DNA reads to the reference genome. So I

Re: [Bioc-devel] Question about external algorithms to Bioconductor package

2017-11-24 Thread Benilton Carvalho
Maybe gmapR? 2017-11-24 16:25 GMT-02:00 Ioannis Vardaxis : > Hei, > > Both kalliston and salmon er for RNA data, I have DNA data. Is there any > other solution rather than Rsubread which is extremely slow? > I am making an algorithm where one of its steps should be to map the DNA > reads to the r

Re: [Bioc-devel] Question about external algorithms to Bioconductor package

2017-11-24 Thread Martin Morgan
On 11/24/2017 09:57 AM, Ioannis Vardaxis wrote: Hi, I tried the Rsubread package you suggested and the mapping is running. However it takes like forever to end. Even in parallel it needs some days to run while bowtie for example needs only a couple of hours in 4 cores. Is there any way of speedi

Re: [Bioc-devel] Question about external algorithms to Bioconductor package

2017-11-24 Thread Ioannis Vardaxis
Hei, Both kalliston and salmon er for RNA data, I have DNA data. Is there any other solution rather than Rsubread which is extremely slow? I am making an algorithm where one of its steps should be to map the DNA reads to the reference genome. So I would like for the user-convenience to do it in my

Re: [Bioc-devel] Question about external algorithms to Bioconductor package

2017-11-24 Thread Ioannis Vardaxis
Hi, I tried the Rsubread package you suggested and the mapping is running. However it takes like forever to end. Even in parallel it needs some days to run while bowtie for example needs only a couple of hours in 4 cores. Is there any way of speeding up Rsubread? Or else I don¹t see any reason usi

Re: [Bioc-devel] Question about external algorithms to Bioconductor package

2017-11-13 Thread A.E.S.
On Sun, 12 Nov 2017 22:22:56 + Ryan Thompson wrote: > Hi, > > I don't know the Bioconductor policy for packages that rely on > external tools, but for the specific features you mention, there are > Bioconductor packages to accomplish most or all of them. You can use > samtools via Rsamtools,

Re: [Bioc-devel] Question about external algorithms to Bioconductor package

2017-11-12 Thread Ryan Thompson
Hi, I don't know the Bioconductor policy for packages that rely on external tools, but for the specific features you mention, there are Bioconductor packages to accomplish most or all of them. You can use samtools via Rsamtools, you can use the Rsubread package in place of bowtie for alignment, an