On 11/24/2017 09:57 AM, Ioannis Vardaxis wrote:
Hi,

I tried the Rsubread package you suggested and the mapping is running.
However it takes like forever to end. Even in parallel it needs some days
to run while bowtie for example needs only a couple of hours in 4 cores.
Is there any way of speeding up Rsubread? Or else I don¹t see any reason
using it, and this is a big problem if I cannot use bowtie inside a
bioconductor package.

I'm not following this thread closely but there are two Bowtie implementations in Bioconductor

http://bioconductor.org/packages/release/bioc/html/Rbowtie.html
http://bioconductor.org/packages/release/bioc/html/Rbowtie2.html

The fast solution for many problems (mapping to known transcripts) is kalisto / salmon, which are not available in Bioconductor -- integrating either of these as _libraries_ would be a nice package.

Martin



Thanks



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