[ccp4bb] Guard columns from FPLC

[Anita P]

Hi All,
Sorry for this off topic.

I have heard that there are these little columns called guard columns which
can be attached to AKTA purifiers. These columns prevent the incoming huge
aggregates to be deposited and blocking of the gel filtration columns.

Can any one advice me regarding where to purchase these columns. I could
not find them in GE website. We have Superdex 16/60 on AKTA purifier.

Thanks in advance.
Have a good day

Anita

[ccp4bb] Announcement of Workshop "Fragment-Screening by Crystallography"

[Müller , Uwe]

Dear all,
as a satellite to the 2014 HZB User Meeting, which will take place from
Dec 03-05, 2014 in Berlin, we are organizing a one-day workshop on
"Fragment-Screening by Crystallography". We assembled a number
of highlight presentations from both academia and industry in order to
give an overview of the field, its current bottlenecks and developments.
For more information on the workshop, please visit:

http://www.helmholtz-berlin.de/events/fragment-screening/index_de.html

The workshop will take place on Dec 03, from 9 am to 6 pm. Registration
is required but free of charge. However, due to space constraints, we have
to limit the number of participants. So register quickly to reserve your place.

Many workshop participants may also want to consider attending the
HZB User Meeting. The Science day will take place on Dec 04, followed
by the typical and popular Berliner Dinner and the X-ray Day will take
place on Dec 05. For more information on the user meeting, including
poster submission please visit:

http://www.helmholtz-berlin.de/user/usermeetings/user-meeting-2014/index_en.html

We are very much looking forward to welcoming you in Berlin.

Uwe & Manfred
Dr. Uwe Mueller
Soft Matter and Functional Materials
Macromolecular Crystallography (BESSY-MX) | Group leader
Elektronenspeicherring BESSY II
Albert-Einstein-Str. 15, D-12489 Berlin, Germany

Fon: +49 30 8062 14974
Fax: +49 30 8062 14975
url: www.helmholtz-berlin.de/bessy-mx
email:u...@helmholtz-berlin.de




Helmholtz-Zentrum Berlin für Materialien und Energie GmbH

Mitglied der Hermann von Helmholtz-Gemeinschaft Deutscher Forschungszentren e.V.

Aufsichtsrat: Vorsitzender Prof. Dr. Dr. h.c. mult. Joachim Treusch, stv. 
Vorsitzende Dr. Beatrix Vierkorn-Rudolph
Geschäftsführung: Prof. Dr. Anke Rita Kaysser-Pyzalla, Thomas Frederking

Sitz Berlin, AG Charlottenburg, 89 HRB 5583

Postadresse:
Hahn-Meitner-Platz 1
D-14109 Berlin

http://www.helmholtz-berlin.de

Re: [ccp4bb] Guard columns from FPLC

[Christian Roth]

Hi Anita,

never heared about Guard columns for Superdex columns. These guard 
columns are usually for HPLC columns (RP) or Silica High pressure 
gelfiltration columns like from Tosoh Bioscience and part of the acutal 
column itself, though replaceable.


Christian

Am 16.09.2014 09:29, schrieb Anita P:

Hi All,
Sorry for this off topic.

I have heard that there are these little columns called guard columns
which can be attached to AKTA purifiers. These columns prevent the
incoming huge aggregates to be deposited and blocking of the gel
filtration columns.

Can any one advice me regarding where to purchase these columns. I could
not find them in GE website. We have Superdex 16/60 on AKTA purifier.

Thanks in advance.
Have a good day

Anita

Re: [ccp4bb] Guard columns from FPLC

[Nadir T. Mrabet]

Well, Pharmacia (now, GE) used to sell such a guard column for FPLC 
prior to Akta.
And I remember buying adaptors to be able to connect the guard column 
and FPLC columns to an HPLC system.

So all the tools exist.
But keep in mind that a guard column volume must in all cases be kept 
minimal to avoid sample dilution which can be deleterious to resolution 
in size-exclusion chromatography.
Prior to FPLC with (or without) a guard column, I have always used an 
Eppendorf microcentrifuge followed by 2u-filtering prior to injecting 
the sample and have consistently observed that these remove most 
aggregates if not all.

HTH,

Nadir Mrabet

Pr. Nadir T. Mrabet
Structural & Molecular Biochemistry
N-gere - INSERM U-954
University of Lorraine, Nancy
School of Sciences and Technologies
& School of Medicine
9, Avenue de la Foret de Haye, BP 184
54505 Vandoeuvre-les-Nancy Cedex
France
Phone: +33 (0)3.83.68.32.73
Fax:   +33 (0)3.83.68.32.79
E-mail: Nadir.Mrabet  univ-lorraine.fr
Cell.: +33 (0)6.11.35.69.09

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On 16/09/2014 19:01, Christian Roth wrote:

Hi Anita,

never heared about Guard columns for Superdex columns. These guard 
columns are usually for HPLC columns (RP) or Silica High pressure 
gelfiltration columns like from Tosoh Bioscience and part of the 
acutal column itself, though replaceable.


Christian

Am 16.09.2014 09:29, schrieb Anita P:

Hi All,
Sorry for this off topic.

I have heard that there are these little columns called guard columns
which can be attached to AKTA purifiers. These columns prevent the
incoming huge aggregates to be deposited and blocking of the gel
filtration columns.

Can any one advice me regarding where to purchase these columns. I could
not find them in GE website. We have Superdex 16/60 on AKTA purifier.

Thanks in advance.
Have a good day

Anita

Re: [ccp4bb] Guard columns from FPLC

[Scott Pegan]

Anita,

>From your description, you most likely are looking for a prefilter6000.

http://www.gelifesciences.com/webapp/wcs/stores/servlet/productById/en/GELifeSciences/18458201

Scott


Scott D. Pegan, Ph.D.
Associate Professor
Pharmaceutical and Biomedical Sciences
College of Pharmacy
University of Georgia
240 W. Green St.
Athens, GA, USA, 30602
phone: 706-542-3435

On Tue, Sep 16, 2014 at 4:29 AM, Anita P  wrote:

> Hi All,
> Sorry for this off topic.
>
> I have heard that there are these little columns called guard columns
> which can be attached to AKTA purifiers. These columns prevent the incoming
> huge aggregates to be deposited and blocking of the gel filtration columns.
>
> Can any one advice me regarding where to purchase these columns. I could
> not find them in GE website. We have Superdex 16/60 on AKTA purifier.
>
> Thanks in advance.
> Have a good day
>
> Anita
>

Re: [ccp4bb] Guard columns from FPLC

[Katherine Sippel]

I think the typical Akta configuration has the prefilter installed after
the mixing pump, but before the sample loop. That is where it is on mine at
least. It wouldn't stop sample precipitate from loading on the column.
Technically, it would probably still work if you installed the prefilter
after the sample loop, but that would add to the dilution problem stated
earlier.

As mentioned by a previous commenter, I personally spin filter everything
right before I load it. That is usually sufficient unless the buffer change
is causing the protein to crash out. Of course if that is the case a guard
column not the solution.

Cheers,
Katherine


On Tue, Sep 16, 2014 at 12:46 PM, Scott Pegan 
wrote:

> Anita,
>
> From your description, you most likely are looking for a prefilter6000.
>
>
> http://www.gelifesciences.com/webapp/wcs/stores/servlet/productById/en/GELifeSciences/18458201
>
> Scott
>
>
> Scott D. Pegan, Ph.D.
> Associate Professor
> Pharmaceutical and Biomedical Sciences
> College of Pharmacy
> University of Georgia
> 240 W. Green St.
> Athens, GA, USA, 30602
> phone: 706-542-3435
>
> On Tue, Sep 16, 2014 at 4:29 AM, Anita P  wrote:
>
>> Hi All,
>> Sorry for this off topic.
>>
>> I have heard that there are these little columns called guard columns
>> which can be attached to AKTA purifiers. These columns prevent the incoming
>> huge aggregates to be deposited and blocking of the gel filtration columns.
>>
>> Can any one advice me regarding where to purchase these columns. I could
>> not find them in GE website. We have Superdex 16/60 on AKTA purifier.
>>
>> Thanks in advance.
>> Have a good day
>>
>> Anita
>>
>
>


-- 
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