Sorry, I'm a little lost as to what problem we are trying to solve. In your first post, you included some warnings, but I'm not sure if those warnings are actual errors. Can you restate your problem?

On 3/17/2025 11:24 AM, Tommaso Mariani wrote:

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The error occurred just immediately after having loaded the surfaces in freeview. There have been no modifications as the output is the one that is given straight out of recon-all command. The issue is encountered both in freesurfer 7.2.0 and 7.4.1 versions.

For what concern the wm edits, I performed some volume edits hoping to fix the hypo- and hyper-intensity problems, but it is more of a side question with respect to the main problem, that is the misregistration of the surfaces.

As for the offset, I mean that the surfaces are not overlapping the volume images, not the orig.mgz nor the wm.mgz ones, giving me the feeling that there is a misregistration problem. I tried to treat it as a tailarach.xfm error, so after following the tutorial I performed the command
tkregisterfv --s ./subj01 --fstal
Though the recon-all launched after this did not produce anything different from the beginning.

I tried the command
mri_convert --conform ./subj01/mri/orig.mgz orig_fixed.mgz
And it gives me as output the message:

input_transform(): error reading transform elem [1,1]
input_transform: error reading transform
error: error loading transform from <path_to_file>/talairach.xfm

The xform info prints out this matrix:
x_r = -1.0000  y_r = 0.0000  z_r = 0.0000  c_r = 0.9241
x_a = 0.0000 y_a = 0.0000 z_a = 1.0000 c_a = -5.7364
x_s = 0.0000  y_s = -1.0000  z_s = -0.0000  c_s = -19.9210

If you need anything else please reach me out.
I am available also to schedule a brief call to find out what's going on, as this issue is what keeps me apart from being able to use the brain model for an important project.

Thank you in advance,
Tommaso Mariani

Il Lun 17 Mar 2025, 14:56 Douglas N. Greve <dgr...@mgh.harvard.edu> ha scritto:

    Did that error happen before you did anything to the volume? Can
    you give a list of commands you used to get to that point? What do
    you mean by offset?

    On 3/10/2025 4:23 AM, Tommaso Mariani wrote:

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    Hi everyone,
    I am experiencing a tricky situation in freesurfer. Due to hypo-
    and hyper-intensity problems I obtained a bad reconstruction and
    I've been working on it since. My first question is then: do I
    need to refresh the aseg file after the wm.mgz intensities
    modifications?

    However I also noticed that when I loaded the surface to verify
    the corrections they were all with a significant offset and this
    message was printed on the console:
    Warning: MatrixInverse failed.
    Did not find any volume info
    Warning: MatrixInverse failed.
    Did not find any volume info
    Warning: MatrixInverse failed.
    Did not find any volume info
    Warning: MatrixInverse failed.
    Did not find any volume info

    Together with that, I was also unable to obtain the final
    parcellization, possibly due to the absence of correlation
    between the volumes and surfaces.
    I already tried to manually adjust the offset and relaunch the
    reconstruction, as suggested in the Freesurfer documentation for
    tailarach errors, but it looks like it did nothing to it.

    If you need any file or info to examine the situation further,
    feel free to contact me, I would really appreciate your help.
    Thanks in advance,
    Tommaso Mariani


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