Here is the tutorial for longitudinal processing - https://surfer.nmr.mgh.harvard.edu/fswiki/LongitudinalProcessing
The 'base' and 'long' steps of the longitudinal processing should be re-run after adding new timepoints. Yujing From: freesurfer-boun...@nmr.mgh.harvard.edu <freesurfer-boun...@nmr.mgh.harvard.edu> On Behalf Of Eszter Boros Sent: Wednesday, January 15, 2025 1:52 PM To: Freesurfer support list <freesurfer@nmr.mgh.harvard.edu> Subject: [Freesurfer] longitudinal analyses, more than 2 timepoints External Email - Use Caution Dear FreeSurfer Team, We are analyzing some longitudinal data (baseline [0], 6 months, 1 year, 1.5 years) using FreeSurfer 7.4.1. We already analyzed two timepoints (basline[0] and 6 months) but now we are trying to look at 4 timepoints in total. I was wondering what the best way would be to do this. For the longitudinal processing, should we create 3 bases/within-subject templates between 0-6 months (this is already done), 0-1year, 0-1.5years), or between 0-6months, 6months-1year, 1year-1.5years. Or both? Also, is it okay to correct/edit only the bases? After the longitudinal processing, can we create a Longitudinal Table and apply the two-stage model (LongitudinalTwoStageModel) method? Or because of the 4 time points, we need to apply something else. Thank you for your help in advance! All the best, Eszter
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