External Email - Use Caution Hi Dr. Doug,
Thanks for your prompt reply! I’m sorry it was not clear. Basically, I would like to extract the cortical volume for each subject using a pre-defined ROI. As I have already created the surface ROI, thanks to your answer on the official website, I have no further question now on ROI. This group analysis can’s further survive the multiple correction using the mri_glmfit-sim that you gave me years ago, so there is no file like cache.th40.neg.sig.ocn.annot as before. Instead, I tried to create a ROI and run command line as $mri_segstats --seg mask1.sm1.bin.mgh --exlude 0 --i groupAnalysis/lh.volume.g2v2.10.mgh --avgwf lh.volume.mask1.dat, where mask1.sm1.bin.mgh is the ROI and groupAnalysis/lh.volume.g2v2.10.mgh is the mris_proc processed file from a group of subjects. It did extract something, 1 number for each subject, but I believe there is a ratio or scalar between the actual volume of that vertex and those show in lh.volume.g2v2.10.mgh. Is there anywhere I can figure out where this ratio/scalar is? Thank you so much! Regards, Xiaoxu From: freesurfer-boun...@nmr.mgh.harvard.edu <freesurfer-boun...@nmr.mgh.harvard.edu> On Behalf Of Douglas N. Greve Sent: Friday, January 19, 2024 3:16 PM To: freesurfer@nmr.mgh.harvard.edu Subject: Re: [Freesurfer] FW: Create a Binarized ROI for Surface On 1/19/2024 3: 45 PM, Na, Xiaoxu wrote: External Email - Use Caution Dear Freesurfer Expert, While the sphere can only be drawn using that Dr. Doug suggested, the sphere option would mess up. Do you need help with this? If so, can you give ZjQcmQRYFpfptBannerStart This Message Is From an External Sender This message came from outside your organization. ZjQcmQRYFpfptBannerEnd On 1/19/2024 3:45 PM, Na, Xiaoxu wrote: External Email - Use Caution Dear Freesurfer Expert, While the sphere can only be drawn using that Dr. Doug suggested, the sphere option would mess up. Do you need help with this? If so, can you give more info? I have a further question as using this ROI to extract surface information from ?h.area and ?h.volume. I’m using the command line as below: $mri_segstats --seg mask1.sm1.bin.mgh --exlude 0 --i groupAnalysis/lh.volume.g2v2.10.mgh --sumwf lh.volume.mask1.dat However, due to the calculation of surface and volume, we need another input of sum file (ASCII file), how can I do that before I can run the above? Sorry, I don't know what you mean Another simple question is, what is lh.volume.mgh in the group analysis folder processed by mris_proc? Is that a simple merge of all subjects lh.volume file? Why the number is between 0-10, is there any way I can put ?h.volume or ?h.area back to gain each subject’s real volume or area value on that surface vertex? Thank you so much! This is a vertex-wise measure of volume of the cortex at that point. Eg, a given vertex has an area defined by the mean area of the triangles that abut it. When this is multiplied by the thickness, you get a volume measure (we actually compute it differently using a truncated pyramid, but that's the basic idea). You can use this in a VBM-like analysis. Regards, Xiaoxu From: Na, Xiaoxu Sent: Wednesday, December 13, 2023 4:25 PM To: Freesurfer support list <freesurfer@nmr.mgh.harvard.edu><mailto:freesurfer@nmr.mgh.harvard.edu> Subject: Create a Binarized ROI for Surface Dear Freesurfer Expert, We would like to extract mean Cth values for each subject within a own-creaded ROI (surface defined), using freeview (for instance). Is it possible to create a ROI (i.e. spherical surface) surrounding a particular vertex of an sig.mgh file? (and then extract its mean Cth values for each subject)? 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These commands did run, but together they didn’t create a binarized mask with 1 for the sphere around the coordinates, and 0 for the other voxels. Would you recommend what I should change? Thank you so much! $mri_volsynth --template $SUBJECTS_DIR/fsaverage/surf/lh.thickness --pdf sphere --sphere-center -7.4 67.22 60.27 --radius 5 --o delta.sm00.mgh $mris_fwhm \ --smooth-only \ --subject fsaverage \ --hemi lh \ --i delta.sm00.mgh \ --niters 10 \ --o delta.sm10.mgh $mri_binarize --i delta.sm10.mgh --min 10e-5 --o delta.sm10.bin.mgh Regards, Xiaoxu ________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. 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