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Dear Bruce,

the ROI appears correct in freeview. However I have a problem in the following 
step, i.e. obtain the centroid of the ROi.

I used the following command:

mri_surfcluster --in 
/Applications/freesurfer/subjects/fsaverage/surf/lh.thickness --clabel 
./tests_label/sub/lh.amygdala.label --sum ./tests_label/sum/sum.lh.amygdala 
--centroid --thmin 0 --hemi lh --subject fsaverage —nofixmni  

However it doesn’t work for subcortical label files (it works with cortical 
ones). I have the following output and the command doesn’t produce the file 
sum.lh.amygdala:

thsign = abs, id = 0
version $Id: mri_surfcluster.c,v 1.57.2.3 2016/11/17 18:19:42 zkaufman Exp $
hemi           = lh
srcid          = /Applications/freesurfer/subjects/fsaverage/surf/lh.thickness 
srcsubjid      = fsaverage
srcsurf        = white
srcframe       = 0
thsign         = abs
thmin          = 0
thmax          = -1
fdr            = -1
minarea        = 0
xfmfile        = talairach.xfm
clabelfile     = ./tests_label/sub/lh.amygdala.label
clabelinv      = 0
nth         = -1
sumfile  = ./tests_label/sum/sum.lh.amygdala
subjectsdir    = /Applications/freesurfer/subjects
FixMNI = 0
Loading clabel ./tests_label/sub/lh.amygdala.label.
Found 1941 points in clabel.
------------- XFM matrix (RAS2RAS) ---------------
/Applications/freesurfer/subjects/fsaverage/mri/transforms/talairach.xfm
 1.00000   0.00000   0.00000   0.00000;
 0.00000   1.00000   0.00000   0.00000;
 0.00000   0.00000   1.00000   0.00000;
 0.00000   0.00000   0.00000   1.00000;
----------------------------------------------------
Reading source surface /Applications/freesurfer/subjects/fsaverage/surf/lh.white
Done reading source surface
Computing metric properties
Loading source values
/Applications/freesurfer/bin/mri_surfcluster: line 3:  2828 Bus error: 10       
    mri_surfcluster.bin "$@"

Maybe because lh.thickness is only for cortical regions? In this case, how can 
I obtain centroids of subcortical ROIs?

Thanks
Regards
Lucia


_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

Dr. Lucia Billeci, PhD
Institute of Clinical Physiology (IFC) National Research Council (CNR)
via Moruzzi 1, 56124, Pisa, Italy
e-mail: lucia.bill...@ifc.cnr.it
_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ 




> On 2 May 2018, at 17:05, Bruce Fischl <fis...@nmr.mgh.harvard.edu> wrote:
> 
> Hi Lucia
> 
> the first column being -1 means that it is not mapped to the surface, so is 
> correct in your (subcortical) case. Try loading the label as an ROI in 
> freeview and see if it is correct
> 
> cheers
> Bruce
> On Wed, 2 May 2018, Lucia Billeci wrote:
> 
>>         External Email - Use Caution        
>> Dear Brouce,
>> thanks again.
>> I would like exactly how to set mri_vol2label for subcortical regions as the 
>> way I used
>> give me all the values of the first column equal to -1. This is a problem 
>> when I want
>> to obtain obtain the centroid of the ROI using mri_surfcluster as I get an 
>> empty file. 
>> Hope Doug can help me.
>> Thanks 
>> Lucia
>> _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _
>> Dr. Lucia Billeci, PhD
>> Institute of Clinical Physiology (IFC) National Research Council (CNR)
>> via Moruzzi 1, 56124, Pisa, Italy
>> e-mail: lucia.bill...@ifc.cnr.it
>> _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ 
>> 
>>      On 2 May 2018, at 16:01, Bruce Fischl <fis...@nmr.mgh.harvard.edu> 
>> wrote:
>> Hi Lucia
>> can you cc the list so others can answer? You can't get a surface-based 
>> label for
>> a subcortical region as they aren't on (or close) to the surrface. I think
>> mri_vol2label is the right binary to create a volumetric ROI for each one, 
>> but
>> Doug will know
>> cheers
>> Bruce
>> On Wed, 2 May 2018, Lucia Billeci wrote:
>> 
>>              External Email - Use Caution        
>>      Dear Bruce,
>>      thanks for your answer.
>>      I would like to obtain .label file for each subcortical  region. I
>>      have tried the commands I
>>      reported in the previous messages but probably they are not correct.
>>      Could you please indicate me
>>      the correct way to have the .label file for subcortical regions? I am
>>      not sure if
>>      using mri_vol2label is the right way or maybe there is another
>>      strategy.
>>      Thanks
>>      regards
>>      Lucia
>>      _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _
>>      Dr. Lucia Billeci, PhD
>>      Institute of Clinical Physiology (IFC) National Research Council
>>      (CNR)
>>      via Moruzzi 1, 56124, Pisa, Italy
>>      e-mail: lucia.bill...@ifc.cnr.it
>>      _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ 
>> The information in this e-mail is intended only for the person to whom it is
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