So you have 1 column, but you want 1 column for each vertex? We don't have anything to do that, but you can do it in matlab, eg,
M = fast_vol2mat(MRIread('lh.yourdata.mgh'));
M will be a 240x127000 matrix

On 5/4/16 8:36 PM, Taha Abdullah wrote:
Hello Doug,

Sorry for the confusing and long email, I am interested in extracting the raw BOLD signal from a processed 4D nifit file. After registering the functional to ${subject}/mri/orig.mgz and generating the registration file, I proceeded to convert the functional image to the same dimensions as the lh.inflated surface via mri_vol2surf so the functional data image now has 127000 x 1 x 1 x 240. So I ran mri_segstats command to extract the bold signal, but I ended up having 240 rows and 1 column in the text file...the ultimate goal is to quantify the wave propagation of the BOLD time series across a flattened patch. Any advice would be greatly appreciated.

Thanks!

On Wed, May 4, 2016 at 2:26 PM, Douglas N Greve <gr...@nmr.mgh.harvard.edu <mailto:gr...@nmr.mgh.harvard.edu>> wrote:


    Sorry, can you tell me what you are trying to do? You just want a
    number
    of time points -by- number of vertices file? Then mri_vol2surf
    should do
    that for you. Flattening is irrelevant for this as it only changes the
    xyz coordinate of the vertex and not the vertex identity.
    doug


    On 04/29/2016 11:24 AM, Taha Abdullah wrote:
    > Hello Freesurfer Experts,
    >
    > Long story short--I would like to extract BOLD values from each TR
    > across all vertices for one subjects flattened surface
    > Following is a brief overview of my steps and at the end you can see
    > where I am stuck.
    > First, after */recon-all /I* followed the steps to cut the lh
    inflated
    > surface, saved as a patch, ran /*mris_flatten, */converted patch
    into
    > asc file.
    > Second, I used read_patch.m to extract all spatial information and a
    > net loss of approximately 10k vertices (127k to 116k)
    >
    > We had all functional images processed in FSL, the 4D file has
    > 64x64x36x240 dimensions (voxels are 3.4x3.4x3.0). Next,
    co-registered
    > the functional and anatomicals together via the following cmd:
    > */ bbregister --s cbp001_v1 --mov filtered_func_data.nii.gz --bold
    > --init-fsl --reg dummy1.da/*t. Afterwards, converted the volume to a
    > surface using the following cmd*/: mri_vol2surf --mov
    > filtered_func_data.nii.gz --reg dummy1.dat --projfrac 0.5 --interp
    > trilinear --hemi lh --o ./lh.func.vol2surf.mgh. /*THe dimensions are
    > 127027 x 1 x 1 x 240. Visually no problem when using*/ tksurfer
    > cbp001_v1 lh inflated -patch /path to flattened patch/ -overlay
    > /lh.func.vol2surf.mgh/  -timecourse lh.func.vol2surf.mgh;/* click on
    > the patch and shows the timecourse for that selected vertex. Using the
    > View>Configure>overlay I can shuffle through the TRs to inspect the
    > change in raw BOLD signal per vertex.
    >
    > I have been perusing the email web server searching for how to
    extract
    > the hemodynamic waveform for each vertex across the flattened
    surface
    > and ultimately will be using matlab to understand how the spatial
    > transformation is happening. As well I have all the matlab files
    that
    > seemed relevant to my query (read_surf.m, read_patch.m, and
    > readMRI.m). I was hoping that I would be able to have a text
    file with
    > all the vertices (127K not the flattened 116k) in rows and each
    column
    > would have the TRs; I ran this command;*/ mri_segstats --slabel
    > cbp001_v1 lh
    > /home/share/freesurfer/subjects/cbp001_v1/label/lh.cortex.label
    > --avgwf mri_seg_stats.dat --i lh.func.vol2surf.mgh/*, output was the
    > 240 TRs as rows and seems like the average global BOLD signal in the
    > single column corresponding with each TR. Excuse my naiveness, I
    just
    > recently (1 month ago) started using freesurfer and I feel like
    I have
    > exhausted as much of the information available on the FS wiki and
    > email server. Any information or advice would be great! I put the
    > commands just to give an idea of my workflow (most of the command
    > lines are from the email server or the FS Wiki) and if there are any
    > issues with my steps please let me know so I can correct them before
    > starting the group analysis.
    >
    > Best,
    > Taha
    >
    > --
    > Taha Abdullah
    > Department of Physiology
    > Northwestern University
    > Masters of Science Physiology and Biophysics, Georgetown
    University 2015
    >
    >
    > _______________________________________________
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    --
    Douglas N. Greve, Ph.D.
    MGH-NMR Center
    gr...@nmr.mgh.harvard.edu <mailto:gr...@nmr.mgh.harvard.edu>
    Phone Number: 617-724-2358 <tel:617-724-2358>
    Fax: 617-726-7422 <tel:617-726-7422>

    Bugs: surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
    <http://surfer.nmr.mgh.harvard.edu/fswiki/BugReporting>
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    www.nmr.mgh.harvard.edu/facility/filedrop/index.html
    <http://www.nmr.mgh.harvard.edu/facility/filedrop/index.html>
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--
Taha Abdullah
Department of Physiology
Northwestern University
Masters of Science Physiology and Biophysics, Georgetown University 2015


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