Hi Bruce,

I am sorry that you could not get me properly.
My query is, how can I preserve direction cosine information. As I am new
to this issue, any help would really be appreciated.

Thanks,
Gunjan

On Wed, Dec 17, 2014 at 7:08 PM, Bruce Fischl <fis...@nmr.mgh.harvard.edu>
wrote:
>
> Hi Gunjan
>
> our coordinate systems are extensively documented here:
>
> http://surfer.nmr.mgh.harvard.edu/fswiki/CoordinateSystems?
> action=AttachFile&do=get&target=fscoordinates.ppt
>
> cheers
> Bruce
>
> On Wed, 17 Dec 2014, Gunjan Gautam wrote:
>
>  Hi Bruce,
>>
>> I can view the file using "freeview -v file.mnc" but I think that won't
>> solve my problem as I need to switch from 2D to 3D and vice versa.
>> I will be thankful if you could be a bit specific about "preserving the
>> direction cosine information" as I am facing difficulty to process these
>> volumes through FS.
>>
>>
>> Thanks,
>> Gunjan
>>
>> On Tue, Dec 16, 2014 at 7:59 AM, Gunjan Gautam <gunjan.gemin...@gmail.com
>> >
>> wrote:
>>
>>       Thank you Bruce,
>>
>>       I try this out.
>>
>>       On Dec 16, 2014 7:58 AM, "Bruce Fischl"
>>       <fis...@nmr.mgh.harvard.edu> wrote:
>>       I'm not sure what to tell you then. You need to save it in
>>       a format that preserves the direction cosine information.
>>       This shouldn't be that hard to do (e.g in matlab)
>>       Bruce
>>
>>
>>       On Tue, 16 Dec 2014, Gunjan Gautam wrote:
>>
>>
>>             I think that won't resolve my issue because I
>>             need to deal with a specific
>>             set of noise removal algorithms and
>>             nonuniformity correction algorithms.
>>
>>             On Dec 16, 2014 7:50 AM, "Bruce Fischl"
>>             <fis...@nmr.mgh.harvard.edu> wrote:
>>                   have you tried just giving the original
>>             volume to recon-all? We
>>                   typically deal with this kind of thing
>>             internally (and in 3D,
>>                   not on a slice-by-slice basis)
>>
>>                   cheers
>>                   Bruce
>>                   On Tue, 16 Dec 2014, Gunjan Gautam
>>             wrote:
>>
>>
>>                         Sure,
>>
>>                         My data(3D volume) is having
>>             different ranges of
>>                         noise and nonuniformity so
>>                         I want to apply different
>>             algorithms to get rid of
>>                         these and analyze the
>>                         extent.
>>
>>                         On Dec 16, 2014 7:40 AM, "Bruce
>>             Fischl"
>>                         <fis...@nmr.mgh.harvard.edu>
>>             wrote:
>>                               Hi Gunjan
>>                               can you elaborate? What kind
>>             of modifications
>>                         would you want to
>>                               make to each slice?
>>
>>                               Bruce
>>                               On Tue, 16 Dec 2014, Gunjan
>>             Gautam wrote:
>>
>>
>>                                     Yes Bruce, minc is a
>>             volume but I want
>>                         to perform
>>                                     some experiments and
>>             see
>>                                     the aftereffects.
>>                                     This is how I come
>>             from 3D to 2D to make
>>                         changes in
>>                                     each slice and again I
>>                                     rejoin these slices
>>             (2D to 3D). I don't
>>                         know if
>>                                     anything other than
>>             NIfTi
>>                                     gives this much
>>             flexibility or it could
>>                         be dealt
>>                                     without coming in 2D
>>             space.
>>
>>                                     Any suggestion will be
>>             highly
>>                         appreciate.
>>
>>                                     On Dec 16, 2014 7:22
>>             AM, "Bruce Fischl"
>>
>>             <fis...@nmr.mgh.harvard.edu> wrote:
>>                                           isn't the minc a
>>             volume? It
>>                         certainly supports
>>                                     holding an entire
>>                                           volume in a
>>             single file
>>                                           On Tue, 16 Dec
>>             2014, Gunjan Gautam
>>                         wrote:
>>
>>
>>                                                 Ok , I try
>>             this out.
>>
>>                                                 I
>>             converted it to nii
>>                         because after
>>                                     processing 2D
>>                                                 slices of
>>             the volume, I
>>                                                 can easily
>>             switch to 3D
>>                         space(ie
>>                                     stacking) in order
>>                                                 to apply
>>             brain
>>                                                 extraction
>>             tools as NIfTi
>>                         provides some
>>                                     of the
>>                                                 useful
>>             utilities.
>>
>>                                                 On Dec 16,
>>             2014 6:59 AM,
>>                         "Bruce Fischl"
>>
>>             <fis...@nmr.mgh.harvard.edu>
>>                         wrote:
>>                                                       why
>>             do you need to
>>                         convert it to
>>                                     nifti? Can
>>                                                 you
>>             process the minc
>>
>>             directly? See if
>>                         freeview can
>>                                     display it:
>>
>>
>>             freeview -v file.mnc
>>
>>
>>
>>                                                       On
>>             Tue, 16 Dec 2014,
>>                         Gunjan Gautam
>>                                     wrote:
>>
>>
>>
>>               Hi Bruce,
>>
>>
>>               Originally, data
>>                         was in MINC
>>                                     format.
>>
>>
>>               On Dec 16, 2014
>>                         1:54 AM,
>>                                     "Bruce Fischl"
>>
>>
>>                         <fis...@nmr.mgh.harvard.edu>
>>                                     wrote:
>>
>>                     Hi Gunjan
>>
>>
>>                     what was
>>                         the original
>>                                     data format
>>                                                 that you
>>
>>               had? Once you
>>                         have
>>
>>                     converted
>>                         it to nifti
>>                                     in your
>>                                                 current
>>             stream
>>
>>               you can no
>>                         longer
>>
>>                     use the
>>                         data, so you
>>                                     have to start
>>                                                 further
>>
>>               upstream
>>
>>
>>                     cheers
>>
>>                     Bruce
>>
>>
>>
>>                     On Tue, 16
>>                         Dec 2014,
>>                                     Gunjan Gautam
>>                                                 wrote:
>>
>>
>>
>>                           I
>>                         don't have
>>                                     dicom for this
>>                                                 subject
>>             but
>>
>>               it can
>>
>>
>>                         easily be
>>                                     converted to from
>>
>>                           .nii
>>                         to .dcm.
>>
>>                           I
>>                         try this out
>>                                     and get back
>>                                                 to you if
>>             I
>>
>>               stuck at
>>
>>                           some
>>                         point.
>>
>>
>>
>>                         Thanks,
>>
>>
>>                         Gunjan
>>
>>
>>                           On
>>                         Dec 16, 2014
>>                                     12:49 AM,
>>                                                 "Bruce
>>             Fischl"
>>
>>
>>
>>             <fis...@nmr.mgh.harvard.edu>
>>                                                 wrote:
>>
>>
>>                           do you
>>                                     have the dicom
>>                                                 data for
>>
>>               this subject?
>>
>>                           If
>>                         so, it will
>>                                     be
>>
>>
>>                           separated
>>                                     into
>>                                                 different
>>             "series"
>>
>>               each
>>
>>
>>                         corresponding to
>>                                     one
>>
>>
>>
>>                                     acquisition (e.g. a
>>
>>             T1-weighted
>>
>>               one such as
>>
>>
>>                         mprage or
>>                                     FLASH).
>>
>>
>>                           Typically
>>                                     that series
>>                                                 will have
>>
>>               more than 150
>>
>>
>>                         slices (since
>>
>>
>>                           that's
>>                                     what it takes
>>                                                 to cover
>>             the
>>
>>               brain at
>>
>>
>>                         close to 1mm
>>                                     voxel
>>
>>
>>                           size). You
>>                                     need to
>>                                                 give
>>             recon-all
>>
>>               the dicom
>>
>>                           file
>>                         that
>>                                     contains a
>>
>>
>>                           single one
>>                                     of those
>>                                                 slices. It
>>
>>               doesn't matter
>>
>>
>>                         which one - we
>>
>>
>>                           will
>>                                     figure out the
>>                                                 rest of
>>             them
>>
>>               from any of
>>
>>                           them
>>
>>
>>
>>                           On Tue, 16
>>                                     Dec 2014,
>>                                                 Gunjan
>>             Gautam
>>
>>               wrote:
>>
>>
>>
>>
>>                                 Hi
>>                                     Bruce,
>>
>>
>>
>>                                 I am
>>                                     sorry to
>>                                                 say that I
>>             did
>>
>>               not get you
>>
>>
>>                         properly.
>>
>>
>>
>>                                     Could you please
>>                                                 say a bit
>>
>>               more about "a
>>
>>
>>                         *single*
>>
>>
>>
>>                                     slice in the
>>                                                 correct
>>
>>
>>
>>                                     series ". I want
>>                                                 to try
>>             this
>>
>>               option.
>>
>>
>>
>>                                 On
>>                                     Dec 16, 2014
>>                                                 12:13 AM,
>>
>>               "Bruce Fischl"
>>
>>
>>
>>
>>             <fis...@nmr.mgh.harvard.edu>
>>
>>               wrote:
>>
>>
>>
>>                                       Hi Gunjan
>>
>>
>>
>>
>>                                       freesurfer
>>                                                 will
>>             handle
>>
>>               the dicom
>>
>>
>>                         slices just
>>
>>
>>
>>                                     find. Just give
>>
>>
>>
>>                                       recon-all
>>                                                 a *single*
>>
>>               slice in the
>>
>>
>>                         correct
>>
>>
>>
>>                                     series with the
>>                                                 -i
>>
>>
>>
>>                                       command
>>                                                 and it
>>             should
>>
>>               work. I
>>
>>
>>                         don't know
>>
>>
>>
>>                                     medcon, but it
>>                                                 is not
>>
>>
>>
>>
>>
>>             propagating the
>>
>>               direction cosine
>>
>>                           info
>>                         into the
>>
>>
>>
>>                                     nifti, which
>>
>>
>>
>>                                       means that
>>                                                 the nifti
>>
>>               files are
>>
>>
>>                         effectively
>>
>>
>>
>>                                     useless as you
>>                                                 will
>>
>>
>>
>>                                       never be
>>                                                 able to
>>
>>               distinguish left
>>
>>                           from
>>                         right
>>
>>
>>
>>                                     again
>>
>>
>>
>>
>>                                       cheers
>>
>>
>>
>>                                       Bruce
>>
>>
>>
>>
>>
>>                                       On Tue, 16
>>                                                 Dec 2014,
>>
>>               Gunjan Gautam
>>
>>
>>                         wrote:
>>
>>
>>
>>
>>
>>
>>                                                 Thanks
>>             Bruce,
>>
>>
>>
>>
>>                                             I
>>                                                 used
>>             "medcon"
>>
>>               command
>>
>>
>>                         (Ubuntu) for the
>>
>>
>>
>>                                     stacking of
>>
>>
>>
>>                                             2D
>>                                                 slices in
>>
>>               order to
>>
>>
>>
>>
>>                                                 convert it
>>             in a
>>
>>               volume.
>>
>>
>>                         These slices
>>
>>
>>                                 were
>>                                     obtained
>>
>>
>>
>>                                             from
>>                                                 a volume
>>
>>               itself and
>>
>>
>>
>>
>>                                                 after
>>             making
>>
>>               some intensity
>>
>>
>>                         changes, a
>>
>>
>>
>>                                     volume is
>>
>>
>>
>>
>>                                                 formed
>>             (using
>>
>>               medcon)
>>
>>
>>
>>
>>                                                 again.
>>             Then I
>>
>>               fed this
>>
>>
>>                         volume as input
>>
>>
>>                                 to
>>                                     FS in
>>
>>
>>
>>
>>                                                 order to
>>             extract
>>
>>               brain.
>>
>>
>>
>>
>>                                             Can
>>                                                 I
>>             overcome
>>
>>               this
>>
>>
>>                         orientation
>>                                     issue ?
>>
>>
>>
>>
>>
>>                                                 Gunjan
>>
>>
>>
>>
>>                                             On
>>                                                 Dec 15,
>>             2014
>>
>>               11:49 PM,
>>
>>
>>                         "Bruce Fischl"
>>
>>
>>
>>
>>
>>
>>                         <fis...@nmr.mgh.harvard.edu>
>>
>>
>>                         wrote:
>>
>>
>>
>>
>>                                                   Hi
>>             Gunjan
>>
>>
>>
>>
>>
>>                                                   the
>>
>>               problem is that
>>
>>                           the
>>                         nifti
>>
>>
>>
>>                                     volume doesn't
>>
>>
>>
>>
>>                                                 contain
>>             the
>>
>>
>>
>>
>>
>>             direction
>>
>>               cosine
>>
>>
>>                         information that
>>
>>
>>                                 we
>>                                     need to
>>
>>
>>
>>
>>                                                 figure out
>>             what
>>
>>
>>
>>
>>
>>             directions
>>
>>               are A/P,
>>
>>                           I/S
>>                         and L/R.
>>
>>
>>
>>                                     mri_convert
>>
>>
>>
>>
>>                                                 warns of
>>             this:
>>
>>
>>
>>
>>
>>
>>
>>               mri_convert
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>             m000-stacks-m000-t1_icbm_normal_1mm_pn0_rf0-00001.nii.gz
>>
>>
>>
>>
>>                                                   test.mgz
>>
>>
>>
>>
>>
>>
>>               mri_convert
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>             m000-stacks-m000-t1_icbm_normal_1mm_pn0_rf0-00001.nii.gz
>>
>>
>>
>>
>>                                                   test.mgz
>>
>>
>>
>>
>>                                                   $Id:
>>
>
>
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