Thank you Bruce,

I try this out.
On Dec 16, 2014 7:58 AM, "Bruce Fischl" <fis...@nmr.mgh.harvard.edu> wrote:

> I'm not sure what to tell you then. You need to save it in a format that
> preserves the direction cosine information. This shouldn't be that hard to
> do (e.g in matlab)
> Bruce
>
>
> On Tue, 16 Dec 2014, Gunjan Gautam wrote:
>
>
>> I think that won't resolve my issue because I need to deal with a specific
>> set of noise removal algorithms and nonuniformity correction algorithms.
>>
>> On Dec 16, 2014 7:50 AM, "Bruce Fischl" <fis...@nmr.mgh.harvard.edu>
>> wrote:
>>       have you tried just giving the original volume to recon-all? We
>>       typically deal with this kind of thing internally (and in 3D,
>>       not on a slice-by-slice basis)
>>
>>       cheers
>>       Bruce
>>       On Tue, 16 Dec 2014, Gunjan Gautam wrote:
>>
>>
>>             Sure,
>>
>>             My data(3D volume) is having different ranges of
>>             noise and nonuniformity so
>>             I want to apply different algorithms to get rid of
>>             these and analyze the
>>             extent.
>>
>>             On Dec 16, 2014 7:40 AM, "Bruce Fischl"
>>             <fis...@nmr.mgh.harvard.edu> wrote:
>>                   Hi Gunjan
>>                   can you elaborate? What kind of modifications
>>             would you want to
>>                   make to each slice?
>>
>>                   Bruce
>>                   On Tue, 16 Dec 2014, Gunjan Gautam wrote:
>>
>>
>>                         Yes Bruce, minc is a volume but I want
>>             to perform
>>                         some experiments and see
>>                         the aftereffects.
>>                         This is how I come from 3D to 2D to make
>>             changes in
>>                         each slice and again I
>>                         rejoin these slices (2D to 3D). I don't
>>             know if
>>                         anything other than NIfTi
>>                         gives this much flexibility or it could
>>             be dealt
>>                         without coming in 2D space.
>>
>>                         Any suggestion will be highly
>>             appreciate.
>>
>>                         On Dec 16, 2014 7:22 AM, "Bruce Fischl"
>>                         <fis...@nmr.mgh.harvard.edu> wrote:
>>                               isn't the minc a volume? It
>>             certainly supports
>>                         holding an entire
>>                               volume in a single file
>>                               On Tue, 16 Dec 2014, Gunjan Gautam
>>             wrote:
>>
>>
>>                                     Ok , I try this out.
>>
>>                                     I converted it to nii
>>             because after
>>                         processing 2D
>>                                     slices of the volume, I
>>                                     can easily switch to 3D
>>             space(ie
>>                         stacking) in order
>>                                     to apply brain
>>                                     extraction tools as NIfTi
>>             provides some
>>                         of the
>>                                     useful utilities.
>>
>>                                     On Dec 16, 2014 6:59 AM,
>>             "Bruce Fischl"
>>                                     <fis...@nmr.mgh.harvard.edu>
>>             wrote:
>>                                           why do you need to
>>             convert it to
>>                         nifti? Can
>>                                     you process the minc
>>                                           directly? See if
>>             freeview can
>>                         display it:
>>
>>                                           freeview -v file.mnc
>>
>>
>>
>>                                           On Tue, 16 Dec 2014,
>>             Gunjan Gautam
>>                         wrote:
>>
>>
>>                                                 Hi Bruce,
>>
>>                                                 Originally, data
>>             was in MINC
>>                         format.
>>
>>                                                 On Dec 16, 2014
>>             1:54 AM,
>>                         "Bruce Fischl"
>>
>>             <fis...@nmr.mgh.harvard.edu>
>>                         wrote:
>>                                                       Hi Gunjan
>>
>>                                                       what was
>>             the original
>>                         data format
>>                                     that you
>>                                                 had? Once you
>>             have
>>                                                       converted
>>             it to nifti
>>                         in your
>>                                     current stream
>>                                                 you can no
>>             longer
>>                                                       use the
>>             data, so you
>>                         have to start
>>                                     further
>>                                                 upstream
>>
>>                                                       cheers
>>                                                       Bruce
>>
>>
>>                                                       On Tue, 16
>>             Dec 2014,
>>                         Gunjan Gautam
>>                                     wrote:
>>
>>
>>                                                             I
>>             don't have
>>                         dicom for this
>>                                     subject but
>>                                                 it can
>>
>>             easily be
>>                         converted to from
>>                                                             .nii
>>             to .dcm.
>>                                                             I
>>             try this out
>>                         and get back
>>                                     to you if I
>>                                                 stuck at
>>                                                             some
>>             point.
>>
>>
>>             Thanks,
>>
>>             Gunjan
>>
>>                                                             On
>>             Dec 16, 2014
>>                         12:49 AM,
>>                                     "Bruce Fischl"
>>
>>                         <fis...@nmr.mgh.harvard.edu>
>>                                     wrote:
>>
>>               do you
>>                         have the dicom
>>                                     data for
>>                                                 this subject?
>>                                                             If
>>             so, it will
>>                         be
>>
>>               separated
>>                         into
>>                                     different "series"
>>                                                 each
>>
>>             corresponding to
>>                         one
>>
>>
>>                         acquisition (e.g. a
>>                                     T1-weighted
>>                                                 one such as
>>
>>             mprage or
>>                         FLASH).
>>
>>               Typically
>>                         that series
>>                                     will have
>>                                                 more than 150
>>
>>             slices (since
>>
>>               that's
>>                         what it takes
>>                                     to cover the
>>                                                 brain at
>>
>>             close to 1mm
>>                         voxel
>>
>>               size). You
>>                         need to
>>                                     give recon-all
>>                                                 the dicom
>>                                                             file
>>             that
>>                         contains a
>>
>>               single one
>>                         of those
>>                                     slices. It
>>                                                 doesn't matter
>>
>>             which one - we
>>
>>               will
>>                         figure out the
>>                                     rest of them
>>                                                 from any of
>>                                                             them
>>
>>
>>               On Tue, 16
>>                         Dec 2014,
>>                                     Gunjan Gautam
>>                                                 wrote:
>>
>>
>>
>>                     Hi
>>                         Bruce,
>>
>>
>>                     I am
>>                         sorry to
>>                                     say that I did
>>                                                 not get you
>>
>>             properly.
>>
>>
>>                         Could you please
>>                                     say a bit
>>                                                 more about "a
>>
>>             *single*
>>
>>
>>                         slice in the
>>                                     correct
>>
>>
>>                         series ". I want
>>                                     to try this
>>                                                 option.
>>
>>
>>                     On
>>                         Dec 16, 2014
>>                                     12:13 AM,
>>                                                 "Bruce Fischl"
>>
>>
>>                                     <fis...@nmr.mgh.harvard.edu>
>>                                                 wrote:
>>
>>
>>                           Hi Gunjan
>>
>>
>>
>>                           freesurfer
>>                                     will handle
>>                                                 the dicom
>>
>>             slices just
>>
>>
>>                         find. Just give
>>
>>
>>                           recon-all
>>                                     a *single*
>>                                                 slice in the
>>
>>             correct
>>
>>
>>                         series with the
>>                                     -i
>>
>>
>>                           command
>>                                     and it should
>>                                                 work. I
>>
>>             don't know
>>
>>
>>                         medcon, but it
>>                                     is not
>>
>>
>>
>>                                     propagating the
>>                                                 direction cosine
>>                                                             info
>>             into the
>>
>>
>>                         nifti, which
>>
>>
>>                           means that
>>                                     the nifti
>>                                                 files are
>>
>>             effectively
>>
>>
>>                         useless as you
>>                                     will
>>
>>
>>                           never be
>>                                     able to
>>                                                 distinguish left
>>                                                             from
>>             right
>>
>>
>>                         again
>>
>>
>>
>>                           cheers
>>
>>
>>                           Bruce
>>
>>
>>
>>
>>                           On Tue, 16
>>                                     Dec 2014,
>>                                                 Gunjan Gautam
>>
>>             wrote:
>>
>>
>>
>>
>>
>>                                     Thanks Bruce,
>>
>>
>>
>>                                 I
>>                                     used "medcon"
>>                                                 command
>>
>>             (Ubuntu) for the
>>
>>
>>                         stacking of
>>
>>
>>                                 2D
>>                                     slices in
>>                                                 order to
>>
>>
>>
>>                                     convert it in a
>>                                                 volume.
>>
>>             These slices
>>
>>                     were
>>                         obtained
>>
>>
>>                                 from
>>                                     a volume
>>                                                 itself and
>>
>>
>>
>>                                     after making
>>                                                 some intensity
>>
>>             changes, a
>>
>>
>>                         volume is
>>
>>
>>
>>                                     formed (using
>>                                                 medcon)
>>
>>
>>
>>                                     again. Then I
>>                                                 fed this
>>
>>             volume as input
>>
>>                     to
>>                         FS in
>>
>>
>>
>>                                     order to extract
>>                                                 brain.
>>
>>
>>
>>                                 Can
>>                                     I overcome
>>                                                 this
>>
>>             orientation
>>                         issue ?
>>
>>
>>
>>
>>                                     Gunjan
>>
>>
>>
>>                                 On
>>                                     Dec 15, 2014
>>                                                 11:49 PM,
>>
>>             "Bruce Fischl"
>>
>>
>>
>>
>>             <fis...@nmr.mgh.harvard.edu>
>>
>>             wrote:
>>
>>
>>
>>                                       Hi Gunjan
>>
>>
>>
>>
>>                                       the
>>                                                 problem is that
>>                                                             the
>>             nifti
>>
>>
>>                         volume doesn't
>>
>>
>>
>>                                     contain the
>>
>>
>>
>>                                       direction
>>                                                 cosine
>>
>>             information that
>>
>>                     we
>>                         need to
>>
>>
>>
>>                                     figure out what
>>
>>
>>
>>                                       directions
>>                                                 are A/P,
>>                                                             I/S
>>             and L/R.
>>
>>
>>                         mri_convert
>>
>>
>>
>>                                     warns of this:
>>
>>
>>
>>
>>
>>                                                 mri_convert
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>             m000-stacks-m000-t1_icbm_normal_1mm_pn0_rf0-00001.nii.gz
>>
>>
>>
>>                                       test.mgz
>>
>>
>>
>>
>>                                                 mri_convert
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>             m000-stacks-m000-t1_icbm_normal_1mm_pn0_rf0-00001.nii.gz
>>
>>
>>
>>                                       test.mgz
>>
>>
>>
>>                                       $Id:
>>                                                 mri_convert.c,v
>>
>>             1.213
>>
>>
>>                         2014/07/29
>>                                     19:22:31
>>
>>
>>
>>                                     fischl Exp $
>>
>>
>>
>>                                       reading
>>                                                 from
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>             m000-stacks-m000-t1_icbm_normal_1mm_pn0_rf0-00001.nii.gz...
>>
>>
>>
>>                                       WARNING:
>>                                                 neither
>>
>>             NIfTI-1 qform or
>>
>>
>>                         sform are
>>
>>
>>
>>                                     valid
>>
>>
>>
>>                                       WARNING:
>>                                                 your volume
>>                                                             will
>>             probably
>>
>>                     be
>>
>>
>>
>>                                     incorrectly
>>                                                 oriented
>>
>>
>>
>>                                       TR=1.00,
>>                                                 TE=0.00,
>>
>>             TI=0.00, flip
>>
>>
>>                         angle=0.00
>>
>>
>>
>>                                       WARNING:
>>                                                 it does not
>>
>>             appear that
>>
>>
>>                         there was
>>
>>
>>
>>                                     sufficient
>>
>>
>>
>>
>>                                                 information
>>
>>
>>
>>                                       in the
>>                                                 input to assign
>>
>>             orientation
>>
>>                     to
>>                         the
>>
>>
>>
>>                                     volume...
>>
>>
>>
>>                                       i_ras =
>>                                                 (-1, 0, 0)
>>
>>
>>
>>                                       j_ras =
>>                                                 (0, 1, 0)
>>
>>
>>
>>                                       k_ras =
>>                                                 (0, 0, 1)
>>
>>
>>
>>                                       writing to
>>                                                 test.mgz...
>>
>>
>>
>>
>>                                       and if you
>>                                                 try to view
>>                                                             the
>>
>>
>>                         converted volume
>>                                     in
>>
>>
>>
>>                                     freeview it
>>                                                 shows
>>
>>
>>
>>                                       up in the
>>                                                 wrong
>>
>>             orientation (e.g
>>
>>                     what
>>                         we think
>>
>>
>>                                 is
>>                                     "coronal" is
>>
>>
>>
>>                                       actually
>>                                                 horizonatal).
>>
>>
>>
>>
>>                                       How did
>>                                                 you create the
>>
>>             nifti
>>
>>
>>                         files? If you
>>
>>
>>                                 give
>>                                     dicom
>>                                                 directly
>>
>>
>>
>>                                       to
>>                                                 recon-all you
>>             will
>>
>>             likely not
>>
>>                     have
>>                         this
>>
>>
>>
>>                                     problem
>>
>>
>>
>>
>>                                       cheers
>>
>>
>>
>>                                       Bruce
>>
>>
>>
>>
>>
>>                                       On Thu, 11
>>                                                 Dec 2014,
>>
>>             Gunjan Gautam
>>
>>
>>                         wrote:
>>
>>
>>
>>
>>                                             ​Dear
>>                                                 all,
>>
>>
>>
>>
>>                                             I am
>>                                                 facing few
>>
>>             errors in
>>
>>
>>                         recon-all
>>
>>
>
>
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