If your label is in diffusion space, then replace the MNI brain with any 
volume that's in diffusion space.

On Fri, 5 Sep 2014, Emily Louise Belleau wrote:

> Hi Anastasia,
>
> This would be in diffusion space though. Since I converted all of my seeds to 
> diffusion space.
>
> Emily
> ________________________________________
> From: freesurfer-boun...@nmr.mgh.harvard.edu 
> <freesurfer-boun...@nmr.mgh.harvard.edu> on behalf of Anastasia Yendiki 
> <ayend...@nmr.mgh.harvard.edu>
> Sent: Friday, September 05, 2014 11:32 AM
> To: Freesurfer support list
> Subject: Re: [Freesurfer] Tracula
>
> To view your label over the MNI average brain:
>
> fslview $FSLDIR/data/standard/MNI152_T1_1mm.nii.gz 
> your_label_in_MNI_space.nii.gz -l Red
>
> There are no settings, other than choosing a different color for the
> label.
>
> On Fri, 5 Sep 2014, Emily Louise Belleau wrote:
>
>> Hi Anastasia,
>>
>> I am still having a bit of trouble understanding how to properly view my 
>> tract through fsl view.
>>
>> What is the appropriate underlay to use/settings?
>>
>> Thanks again for your continued help!
>>
>> Emily
>> ________________________________________
>> From: freesurfer-boun...@nmr.mgh.harvard.edu 
>> <freesurfer-boun...@nmr.mgh.harvard.edu> on behalf of Anastasia Yendiki 
>> <ayend...@nmr.mgh.harvard.edu>
>> Sent: Thursday, September 04, 2014 4:54 PM
>> To: Freesurfer support list
>> Subject: Re: [Freesurfer] Tracula
>>
>> Certainly you can, and it's a good idea for troubleshooting this. In FSL
>> you can do it with:
>>
>> flirt -in your_label_in_MNI_space.nii.gz -out 
>> your_label_in_diff_space.nii.gz -ref dmri/lowb.nii.gz -applyxfm -init 
>> dmri/xfms/mni2diff.bbr.mat -interp nearestneighbour
>>
>> Then you can overlay the transformed label on some of the other inputs
>> that you're giving probtrackx (e.g. a volume from the bedpostx directory)
>> to make sure they look right in fslview.
>>
>>
>> On Thu, 4 Sep 2014, Emily Louise Belleau wrote:
>>
>>> Hi Anastasia,
>>>
>>> Yes my amygdala seed is in MNI space. When I used the MNI to diffusion.mat 
>>> file in the dmri folder and placed it into FSL's probtractx, I developed a 
>>> track that was in the wrong area of the brain, so I am thinking there is 
>>> something wrong happening with the transformation to diffusion space.
>>>
>>> Can I transform the amygdala mask into diffusion space prior to putting it 
>>> into FSL's probtrackx viz bb register. If so, how might I go about doing 
>>> that?
>>>
>>> Thanks so much for your help,
>>>
>>> Emily
>>> ________________________________________
>>> From: freesurfer-boun...@nmr.mgh.harvard.edu 
>>> <freesurfer-boun...@nmr.mgh.harvard.edu> on behalf of Anastasia Yendiki 
>>> <ayend...@nmr.mgh.harvard.edu>
>>> Sent: Thursday, September 04, 2014 4:26 PM
>>> To: Freesurfer support list
>>> Subject: Re: [Freesurfer] Tracula
>>>
>>> Hi Emily - Is your amygdala seed ROI in MNI space? Only in that case the
>>> seed-to-diffusion transformation would be the MNI-to-diffusion
>>> transformation.
>>>
>>> a.y
>>>
>>> On Wed, 3 Sep 2014, Emily Louise Belleau wrote:
>>>
>>>> Hello,
>>>>
>>>> I did all of my DTI preprocessing through tracula.  I now am using FSL 
>>>> probtractx (which utilizes the bedpost stage output) to explore pathways 
>>>> from the amygdala to the ventromedial prefontal cortex.
>>>>
>>>> When I used FSL's probtractx, when I viewed the tract. The tract was in 
>>>> the wrong area of the brain.
>>>>
>>>> So, I think this may have something to do with the transfromation matrices 
>>>> I gave it via tracula.
>>>>
>>>> *FSL asks for the seed to diffusion transformation matrix so that it can 
>>>> convert your seed masks from MNI space to diffusion space. I gave the 
>>>> transformation matrix  in the dmri folder under xfms, titled 
>>>> mni2diff.bbr.mat. I have a feeling this may be the issue.
>>>>
>>>> * I then tried to use the FLIRT registration tool through FSL to get the 
>>>> appropriate transformation matrices.  It asks for the bedpost directory. I 
>>>> got an error saying it could not read my no_dif_brain_mask.nii.
>>>>
>>>> I am not sure what transformation matrix to give and was wondering if you 
>>>> could give me some guidance.
>>>>
>>>> Thanks,
>>>>
>>>> Emily
>>>>
>>>>
>>>> _______________________________________________
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>>>> https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer
>>>>
>>>>
>>>>
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