Hi Emily - Is your amygdala seed ROI in MNI space? Only in that case the 
seed-to-diffusion transformation would be the MNI-to-diffusion 
transformation.

a.y

On Wed, 3 Sep 2014, Emily Louise Belleau wrote:

> Hello,
>
> I did all of my DTI preprocessing through tracula.  I now am using FSL 
> probtractx (which utilizes the bedpost stage output) to explore pathways from 
> the amygdala to the ventromedial prefontal cortex.
>
> When I used FSL's probtractx, when I viewed the tract. The tract was in the 
> wrong area of the brain.
>
> So, I think this may have something to do with the transfromation matrices I 
> gave it via tracula.
>
> *FSL asks for the seed to diffusion transformation matrix so that it can 
> convert your seed masks from MNI space to diffusion space. I gave the 
> transformation matrix  in the dmri folder under xfms, titled 
> mni2diff.bbr.mat. I have a feeling this may be the issue.
>
> * I then tried to use the FLIRT registration tool through FSL to get the 
> appropriate transformation matrices.  It asks for the bedpost directory. I 
> got an error saying it could not read my no_dif_brain_mask.nii.
>
> I am not sure what transformation matrix to give and was wondering if you 
> could give me some guidance.
>
> Thanks,
>
> Emily
>
>
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