Hello,
I suppose you have good data, calculate also an anomalous Fourier
synthesis, there should be an anomalous signal for the calcium, even as
it might be very small.
See https://www.ruppweb.org/Xray/elements.html
Wim
Le 10/09/2025 à 15:22, Tjaard Pijning a écrit :
Dear community,
I have a so far unexplained density near two Asp’s and one Asn on the
surface of my protein (a bacterial enzyme). It could be connected to
the Asp residues but I don’t know of any posttranslational
modification (if that’s what it is) that would fit. I’ve put three
different views in the attachment with blue 2Fo-Fc contoured at 1.0
sigma, and green/red Fo-Fc at +3.0/-3.0 sigma.
The crystallization components are PEG 20k, glycerol, MES,
BisTrisPropane, NaCl and CaCl2.
Any help/advice is greatly appreciated!
With kind regards,
Tjaard
*Dr. Ing. Tjaard Pijning
***
Biomolecular X-ray Crystallography
Faculty of Science and Engineering**
University of Groningen
Nijenborgh 7, 9747 AG Groningen
The Netherlands
Office 5171.00.70 (Mo-Thu)
Tel +31 6 3192 1948
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