I initially thought of glycerol but it would be strange having them interacting
with these negative side chains. Could you run a Mass Spec of your gel? It
should be easy to identify everything bound to this region of the protein.
Best wishes
______________________________________________________
Rafael Marques da Silva
PhD Student – Structural Biology
University of Leicester
Mestre em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal de São Carlos
phone: +44 07861 273773
"A sorte acompanha uma mente bem treinada"
________________________________________________
________________________________
De: CCP4 bulletin board <[email protected]> em nome de Tjaard Pijning
<[email protected]>
Enviado: quarta-feira, 10 de setembro de 2025 14:22
Para: [email protected] <[email protected]>
Assunto: [ccp4bb] Density identification
Dear community,
I have a so far unexplained density near two Asp’s and one Asn on the surface
of my protein (a bacterial enzyme). It could be connected to the Asp residues
but I don’t know of any posttranslational modification (if that’s what it is)
that would fit. I’ve put three different views in the attachment with blue
2Fo-Fc contoured at 1.0 sigma, and green/red Fo-Fc at +3.0/-3.0 sigma.
The crystallization components are PEG 20k, glycerol, MES, BisTrisPropane, NaCl
and CaCl2.
Any help/advice is greatly appreciated!
With kind regards,
Tjaard
Dr. Ing. Tjaard Pijning
Biomolecular X-ray Crystallography
Faculty of Science and Engineering
University of Groningen
Nijenborgh 7, 9747 AG Groningen
The Netherlands
Office 5171.00.70 (Mo-Thu)
Tel +31 6 3192 1948
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