Hi Tjaard, I think these are probably two calcium ions coordinated by water molecules.
Best wishes, Tomas On Wed, Sep 10, 2025 at 2:23 PM Tjaard Pijning <[email protected]> wrote: > > Dear community, > > > > I have a so far unexplained density near two Asp’s and one Asn on the surface > of my protein (a bacterial enzyme). It could be connected to the Asp residues > but I don’t know of any posttranslational modification (if that’s what it is) > that would fit. I’ve put three different views in the attachment with blue > 2Fo-Fc contoured at 1.0 sigma, and green/red Fo-Fc at +3.0/-3.0 sigma. > > > > The crystallization components are PEG 20k, glycerol, MES, BisTrisPropane, > NaCl and CaCl2. > > > > Any help/advice is greatly appreciated! > > > > With kind regards, > > Tjaard > > > > Dr. Ing. Tjaard Pijning > > Biomolecular X-ray Crystallography > Faculty of Science and Engineering > > University of Groningen > > Nijenborgh 7, 9747 AG Groningen > > The Netherlands > > Office 5171.00.70 (Mo-Thu) > > Tel +31 6 3192 1948 > > > > > ________________________________ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
