Hi Dr. Islam, When I first looked at the images you sent it seems you are looking down a crystallographic or non-crystallographic 2-fold symmetry axis. The Fo-Fc map looks symmetrical (2 fold) suggesting perhaps you have two small molecules binding at the interface between the two molecules shown.
Andy Mesecar _______________________________________________________ Andrew D. Mesecar Distinguished Professor of Biochemistry Walther Professor of Cancer Structural Biology Robert W. Miller Director, Purdue Institute for Cancer Research Assistant Vice President for Research E-Mail: amese...@purdue.edu<http://amese...@purdue.edu/> Homepage: https://www.science.purdue.edu/mesecar/index.html Executive Assistant: Jaime Turner – jjtur...@purdue.edu [https://ci3.googleusercontent.com/mail-sig/AIorK4wTxlJh83zvrozRaLp2CxBlXTOe4wjNcUOWja5sPFCt-CBMbzTajsP2gddXxapH5NW7xxxBMDA] _______________________________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of zeyaul islam <zeya1...@gmail.com> Date: Monday, January 6, 2025 at 2:57 AM To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] Unidentified Density You don't often get email from zeya1...@gmail.com. Learn why this is important<https://aka.ms/LearnAboutSenderIdentification> ---- External Email: Use caution with attachments, links, or sharing data ---- Dear all We have solved the structure of c1 domain of cardiac isoform of myosin binding protein-c at 2.1 angstrom. We observe a large unidentified density near the symmetry. Please have a look at the figures. Protein was purified in 50 mM Tris.HCl pH 8, 150 mM NaCl, 1 mM DTT and the crystallisation condition has 0.04 M KPO4, 16% PEG8000 and 20% Glycerol. Any ideas what this could be? Data was collected under cryo condition (glycerol as cryoprotectant) at automated macromolecular crystallography (AMX) /17-ID-1 beamline of National Synchrotron Light Source II (NSLSII) located at Brookhaven National Laboratory (BNL). I appreciate any help you can provide. Thanks Zeya Zeyaul Islam, PhD QBRI - Qatar Biomedical Research Institute P.O. Box: 34110 Doha – Qatar Tel: +974 445 48033 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
