It looks like there is a 2-fold axis through the middle, but thinking of HIV 
protease, that might not stop it being a peptide.

Best wishes, Jon Cooper.
jon.b.coo...@protonmail.com

Sent from Proton Mail Android

-------- Original Message --------
On 06/01/2025 10:26, zeyaul islam  wrote:

> Hi,
> Yes, it seems to be a peptide. Our construct was around 52 kDa but our 
> structure has a 12 kDa fragment as this protein undergoes self-proteolysis. 
> In fact previous structures have this fragment only without additional 
> peptides. looks like a caught cleaved product.
> Protein was expressed in bacteria and purified using affinity and gel 
> filtration chromatography.
>
> Thanks
> Zeya
>
> Zeyaul Islam, PhD
>
> QBRI - Qatar Biomedical Research Institute
>
> P.O. Box: 34110
> Doha – Qatar
>
> Tel: +974 445 48033
>
> On Mon, Jan 6, 2025 at 11:33 AM Tom Peat <t.p...@unsw.edu.au> wrote:
>
>> Hello Zeya,
>>
>> More information may be needed to give the community a better chance at this 
>> question. How was the protein expressed and purified? This could lead to 
>> some clues as to what the density may be.
>> Have you considered that it might be a small peptide?
>> Best regards, tom
>>
>> ---------------------------------------------------------------
>>
>> From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of zeyaul islam 
>> <zeya1...@gmail.com>
>> Sent: Monday, January 6, 2025 6:55 PM
>> To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK>
>> Subject: [ccp4bb] Unidentified Density
>>
>> You don't often get email from zeya1...@gmail.com. [Learn why this is 
>> important](https://aka.ms/LearnAboutSenderIdentification)
>>
>> Dear all
>>
>> We have solved the structure of c1 domain of cardiac isoform of myosin 
>> binding protein-c at 2.1 angstrom. We observe a large unidentified density 
>> near the symmetry. Please have a look at the figures. Protein was purified 
>> in 50 mM Tris.HCl pH 8, 150 mM NaCl, 1 mM DTT and the crystallisation 
>> condition has 0.04 M KPO4, 16% PEG8000 and 20% Glycerol. Any ideas what this 
>> could be?
>>
>> Data was collected under cryo condition (glycerol as cryoprotectant) at 
>> automated macromolecular crystallography (AMX) /17-ID-1 beamline of National 
>> Synchrotron Light Source II (NSLSII) located at Brookhaven National 
>> Laboratory (BNL).
>>
>> I appreciate any help you can provide.
>>
>> Thanks
>>
>> Zeya
>>
>> Zeyaul Islam, PhD
>>
>> QBRI - Qatar Biomedical Research Institute
>>
>> P.O. Box: 34110
>> Doha – Qatar
>>
>> Tel: +974 445 48033
>>
>> ---------------------------------------------------------------
>>
>> To unsubscribe from the CCP4BB list, click the following link:
>> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
>
> ---------------------------------------------------------------
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1

########################################################################

To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1

This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list 
hosted by www.jiscmail.ac.uk, terms & conditions are available at 
https://www.jiscmail.ac.uk/policyandsecurity/

Reply via email to