Hello Zeya, More information may be needed to give the community a better chance at this question. How was the protein expressed and purified? This could lead to some clues as to what the density may be. Have you considered that it might be a small peptide? Best regards, tom ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of zeyaul islam <zeya1...@gmail.com> Sent: Monday, January 6, 2025 6:55 PM To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] Unidentified Density
You don't often get email from zeya1...@gmail.com. Learn why this is important<https://aka.ms/LearnAboutSenderIdentification> Dear all We have solved the structure of c1 domain of cardiac isoform of myosin binding protein-c at 2.1 angstrom. We observe a large unidentified density near the symmetry. Please have a look at the figures. Protein was purified in 50 mM Tris.HCl pH 8, 150 mM NaCl, 1 mM DTT and the crystallisation condition has 0.04 M KPO4, 16% PEG8000 and 20% Glycerol. Any ideas what this could be? Data was collected under cryo condition (glycerol as cryoprotectant) at automated macromolecular crystallography (AMX) /17-ID-1 beamline of National Synchrotron Light Source II (NSLSII) located at Brookhaven National Laboratory (BNL). I appreciate any help you can provide. Thanks Zeya Zeyaul Islam, PhD QBRI - Qatar Biomedical Research Institute P.O. Box: 34110 Doha – Qatar Tel: +974 445 48033 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
