Hello Zeya,

More information may be needed to give the community a better chance at this 
question. How was the protein expressed and purified? This could lead to some 
clues as to what the density may be.
Have you considered that it might be a small peptide?
Best regards, tom
________________________________
From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of zeyaul islam 
<zeya1...@gmail.com>
Sent: Monday, January 6, 2025 6:55 PM
To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK>
Subject: [ccp4bb] Unidentified Density

You don't often get email from zeya1...@gmail.com. Learn why this is 
important<https://aka.ms/LearnAboutSenderIdentification>

Dear all

 We have solved the structure of c1 domain of cardiac isoform of myosin binding 
protein-c at 2.1 angstrom. We observe a large unidentified density near the 
symmetry. Please have a look at the figures. Protein was purified in 50 mM 
Tris.HCl pH 8, 150 mM NaCl, 1 mM DTT and the crystallisation condition has 0.04 
M KPO4, 16% PEG8000 and 20% Glycerol. Any ideas what this could be?

Data was collected under cryo condition (glycerol as cryoprotectant) at  
automated macromolecular crystallography (AMX) /17-ID-1 beamline of National 
Synchrotron Light Source II (NSLSII) located at Brookhaven National Laboratory 
(BNL).

I appreciate any help you can provide.

Thanks

Zeya

Zeyaul Islam, PhD

QBRI - Qatar Biomedical Research Institute

P.O. Box: 34110
Doha – Qatar

Tel: +974 445 48033


________________________________

To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1

########################################################################

To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1

This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list 
hosted by www.jiscmail.ac.uk, terms & conditions are available at 
https://www.jiscmail.ac.uk/policyandsecurity/

Reply via email to