A few years ago, I configured the Opentrons OT-2 robot to create optimization screens by pipetting from conical tubes into deep well blocks. While the setup works well, it does require some fine-tuning, particularly with pipetting speed, to handle solutions that tend to stick to the plastic tips. Before running the method, I would manually test each solution by dipping a tip into it to check for residual drops clinging to the side. If I noticed lingering drops, I adjusted the program to pipette more slowly. All adjustments and calculations were managed via an Excel spreadsheet that also computes the required volumes. The method and corresponding Excel spreadsheet are available here: https://protocols.opentrons.com/protocol/38efd4 The current setup supports up to eight reagents, but with some coding adjustments, it can be scaled to accommodate additional reagents. While this solution is budget-friendly, it’s not the fastest option. Opentrons has released a newer and faster robot, though I haven’t had the chance to try it myself.
Best, Ricardo Ricardo Padua, Ph.D. HHMI Research Specialist (858) 784-8034 https://kernlab-scripps.github.io 10550 North Torrey Pines Road La Jolla, CA 92037 ? [cid:bd819629-644d-4196-a0ae-ae390269af1e]<https://www.scripps.edu/> ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Andy Purkiss <andrew.purk...@crick.ac.uk> Sent: Monday, December 2, 2024 5:48 AM To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: Re: [ccp4bb] Pipetting Robot We generally only use Mitegen In-situ-1 plates with optimisation conditions dispensed with the Formulator not initial screens. However, our Art-Robbins Pheonix (once I repair it) can dispense into these trays from a deep well block (as would the Hydra it replaced as they share a similart head). It would probably work better with the finer flexible needles given the small target. We don't dispense the protein, just aliquot the screen into the reservoir and then move to the NT8 or Mosquito. We have also tried dispensing deep well blocks from tube format using larger more generic liquid handling robots in our high throughput facility. This works fairly well but not all screens behave due to a mix of variable viscosity and surface tension across the conditions. Hope this is of interest. Andy ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Sarah Bowman <0000ad1e26f054b6-dmarc-requ...@jiscmail.ac.uk> Sent: 02 December 2024 13:35 To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: Re: [ccp4bb] Pipetting Robot External Sender: Use caution. We use the Formulator from Formulatrix for mixing cocktails. We use it to mix 96 well deep well blocks for preparing the 1536 high-throughput screen we use in the National Crystallization Center at HWI, as well as for optimization plates. I'm not sure if any of the others can dispense into deep well blocks – if anyone uses another robot to do so, please let me know! I echo Halina's request – does anyone make use of a robot to set up Mitegen in situ plates? Warmly, Sarah Sarah EJ Bowman PhD Associate Investigator | Hauptman-Woodward Medical Research Institute Director | National Crystallization Center | Hauptman-Woodward Medical Research Institute Research Associate Professor | Department of Biochemistry and Department of Structural Biology | University at Buffalo | Jacobs School of Medicine and Biomedical Sciences Member | US National Committee for Crystallography Fellow | American Crystallographic Association – The Structural Science Society e1: sbowman at hwi.buffalo.edu e2: sebowman at buffalo.edu www.getacrystal.org<http://www.getacrystal.org/> Hauptman-Woodward Medical Research Institute 700 Ellicott Street | Buffalo, NY 14203 From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of "Mikolajek, Halina (DLSLtd,RAL,LSCI)" <00009bf9bf19ae9c-dmarc-requ...@jiscmail.ac.uk> Reply-To: "Mikolajek, Halina (DLSLtd,RAL,LSCI)" <halina.mikola...@diamond.ac.uk> Date: Monday, December 2, 2024 at 5:58 AM To: "CCP4BB@JISCMAIL.AC.UK" <CCP4BB@JISCMAIL.AC.UK> Subject: Re: [ccp4bb] Pipetting Robot Hi all, tagging onto this thread. Which robots do people use for the Mitegen Insitu plate for dispensing commercial screens? Ideally, with washing tips, rather than using fresh tips each time. Best wi This sender is trusted. sophospsmartbannerend Hi all, tagging onto this thread. Which robots do people use for the Mitegen Insitu plate for dispensing commercial screens? Ideally, with washing tips, rather than using fresh tips each time. Best wishes Halina Mikolajek ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Ulrike Demmer <ulrike.dem...@biophys.mpg.de> Sent: 02 December 2024 07:53 To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] Pipetting Robot Dear crystallographers, we are thinking about buying a new robot for design and pipetting screens. What instruments do you use ? Any recommendations or experiences you would like to share ? 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