Dear Vasily,
HIC can also be used for lipid removal, either by using resins like
Lipidex or butyl/phenyl Sepharose. Example:
https://doi.org/10.1016/j.jchromb.2008.04.011
Best,
Miha
--
Miha Pavšič
University of Ljubljana, Faculty of Chemistry and Chemical Technology,
Chair of Biochemistry
Večna pot 113, SI-1000 Ljubljana, Slovenia
phone +386 1 479 8550
On 2024-10-22 13:36, Hans Raaijmakers wrote:
Dear Vasily,
If triton x-100 won’t wash the lipids away when the protein is bound to a
column, how about crystallising with a compound that is known to push the lipid
out and continue with (multi-step) soaks for your compound of interest?
Cheers
Hans
Verstuurd vanaf mijn iPhone
Op 22 okt. 2024 om 08:49 heeft Vasily Morozov <vasily.moro...@cup.lmu.de> het
volgende geschreven:
Hi everyone,
I am working on several nuclear receptors that can bind phospholipids in their
hydrophobic pockets (expected Kd around two-digit nanomolar). Do you have any
suggestions for methods to remove the lipids apart from long dialysis? I plan
to use the proteins in co-crystallization experiments with small molecules, and
I'm concerned that the lipids might interfere.
Thanks in advance!
Vasily Morozov
Department of Pharmacy
Ludwig-Maximilians-Universität
Butenandstraße 5-13, Haus C
D-81377 München
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