Dear Vasily, If triton x-100 won’t wash the lipids away when the protein is bound to a column, how about crystallising with a compound that is known to push the lipid out and continue with (multi-step) soaks for your compound of interest?
Cheers Hans Verstuurd vanaf mijn iPhone > Op 22 okt. 2024 om 08:49 heeft Vasily Morozov <vasily.moro...@cup.lmu.de> het > volgende geschreven: > > Hi everyone, > > I am working on several nuclear receptors that can bind phospholipids in > their hydrophobic pockets (expected Kd around two-digit nanomolar). Do you > have any suggestions for methods to remove the lipids apart from long > dialysis? I plan to use the proteins in co-crystallization experiments with > small molecules, and I'm concerned that the lipids might interfere. > > Thanks in advance! > > Vasily Morozov > > Department of Pharmacy > Ludwig-Maximilians-Universität > Butenandstraße 5-13, Haus C > D-81377 München > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing > list hosted by www.jiscmail.ac.uk, terms & conditions are available at > https://www.jiscmail.ac.uk/policyandsecurity/ ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
