Hi everyone, I am working on several nuclear receptors that can bind phospholipids in their hydrophobic pockets (expected Kd around two-digit nanomolar). Do you have any suggestions for methods to remove the lipids apart from long dialysis? I plan to use the proteins in co-crystallization experiments with small molecules, and I'm concerned that the lipids might interfere.
Thanks in advance! Vasily Morozov Department of Pharmacy Ludwig-Maximilians-Universität Butenandstraße 5-13, Haus C D-81377 München ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
