Hi Doeke,
if I remember correctly, D2O is poisonous and bacteria grow with reduced
growth rate.
The effect of the greater mass may not be totally insignificant.
Best,
Tim
Am 06.09.2024 15:25, schrieb Hekstra, Doeke Romke:
Hi Arpita,
H and D have the same number of electrons (1). The D nucleus has an
extra neutron, changing its mass and therefore its vibrational energy
levels. That can affect hydrogen bonding patterns, although I would
expect the effect to be relatively minor (see e.g. Fisher and
Helliwell, Acta Cryst A A64: 359-367 (2008)).
Best, Doeke
From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> On Behalf Of Arpita
Goswami
Sent: Thursday, September 5, 2024 11:12 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Studying hydrogen bonding network by neutron
diffraction in sample with D2O
Dear all,
Good morning, Sorry for the off-topic question.
There is a protein crystal sample in which water bonding networks by
certain species to be studied by neutron diffraction, not exactly
within the protein. But between the subunits of the protein. Now it is
mandatory to use some D2O for the preparation of samples. Now my
question is will there be drastic differences between the hydration
patterns of this sample and original sample without D2O? For example,
some change in positions and interacting partners of the hydrated
species of interest with respect to the protein surface? Since
deuterium has 2 electrons instead of one as in protium?
We know D2O is regularly used for such experiments in Neutron
diffraction and in NMR as well. Please let me know your expert
opinions.
Thank you.
Best Regards,
Arpita
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Tim Gruene
Head of the Centre for X-ray Structure Analysis
Faculty of Chemistry
University of Vienna
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