Hello Dr. Mathew, Good day!
Thank you for the explanation and for correcting the sloppy mistake for Deuterium electron numbers! Regarding pKa, will there be differences then in the hydrogen bonding patterns between H2O and D2O enriched solutions for pH 6 to 7 range? Best regards, Arpita On Fri, Sep 6, 2024, 11:15 Matthew Merski <mer...@gmail.com> wrote: > Deuterium only has one electron, like hydrogen. It has a neutron and a > proton making it an isotope of hydrogen. There will be a difference in > hydrogen bonding depending on pH as water and D2O have slightly different > pKa behaviour, but far from the pKa this will not be noticeable. > > On Fri, 6 Sept 2024, 04:12 Arpita Goswami, <bt.arp...@gmail.com> wrote: > >> Dear all, >> >> Good morning, Sorry for the off-topic question. >> >> There is a protein crystal sample in which water bonding networks by >> certain species to be studied by neutron diffraction, not exactly within >> the protein. But between the subunits of the protein. Now it is mandatory >> to use some D2O for the preparation of samples. Now my question is will >> there be drastic differences between the hydration patterns of this sample >> and original sample without D2O? For example, some change in positions and >> interacting partners of the hydrated species of interest with respect to >> the protein surface? Since deuterium has 2 electrons instead of one as in >> protium? >> >> We know D2O is regularly used for such experiments in Neutron diffraction >> and in NMR as well. Please let me know your expert opinions. >> >> Thank you. >> Best Regards, >> Arpita >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
