I’ve just been playing with a difference map to check something. If you fail to 
set the map as a difference map (either when opening the map initially, or 
using Calculate->Map Tools->Set map is a difference map), there’s nothing in 
the Display Manager->Properties window that tells you. I was wondering if that 
could be the issue, if the contour colour chosen when it isn’t set as a 
difference map doesn’t show up against the other map.

Randy

> On 2 Aug 2023, at 14:32, Andrea Smith <andrea.sm...@uochb.cas.cz> wrote:
> 
> Dear Eleanor,
> 
> yes, I made the printscreen with the setting that shows both maps contoured 
> at 0.08 = 3 rmsd.
> 
> I also attach a printscreen from a site where I believe a Zn ion is bound 
> (from my condition). Can see that Wincoot is drawing the map wrong...
> Andrea
> 
> 
> 
> On Wednesday, August 02, 2023 15:28 CEST, Eleanor Dodson 
> <0000176a9d5ebad7-dmarc-requ...@jiscmail.ac.uk> wrote:
>  
>> 
>> Hmmm -are the two ano maps contoured at the same level? 
>> You can check this by setting the SCROLL option to the ano map.
>>  The 2mFo-DFc maps look pretty identical..which suggests the two input mtz 
>> files are similar.
>> You can get more information by viewing the inputs to make sure the values 
>> are the same..
>> Eleanor
>>   On Wed, 2 Aug 2023 at 13:53, Andrea Smith <andrea.sm...@uochb.cas.cz> 
>> wrote:
>> Dear all,
>> 
>> I used refmac in CCP4Cloud and then I opened the generated .pdb and .mtz 
>> from the CCP4Cloud job both in Linux Coot 0.9.6. and WinCoot 0.9.6. I can 
>> see different anomalous maps in Coot and Wincoot - see attached printscreen 
>> where on the left there is a green electron density between the aspartates 
>> while on the right there is none. I tried to search if this is a bug but 
>> couldn't find info about this.
>> 
>> Am I doing something wrong? Do I have a local software issue?
>> 
>> Any advice would be appreciated, best,
>> Andrea
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> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 <Zn.png>

-----
Randy J. Read
Department of Haematology, University of Cambridge
Cambridge Institute for Medical Research     Tel: +44 1223 336500
The Keith Peters Building
Hills Road                                                       E-mail: 
rj...@cam.ac.uk
Cambridge CB2 0XY, U.K.                              
www-structmed.cimr.cam.ac.uk


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