Hello,
I have a maybe naive question, did you check the results for the Matthew
Probabilities calculation ?
Are you 100% certain that's your protein of interest in the crystal?
Sometimes we can have surprise.
Nicolas
On 03/03/2022 05:43, Shubhashish Chakraborty wrote:
Hello,
I am trying to solve a dataset using molecular replacement. However,
neither Phaser MR nor Molrep can give any solution.
In Phaser, I have received an advisory that Top FTF has not packed.
I have tried molecular replacement using the wild-type protein at
different resolutions (I am working on a mutant).
Also, I have truncated the loops from the input structure. However,
none have worked.
So, what can be the possible way to solve this data set?
Thank you
Shubhashish Chakraborty
PhD JRF 2018
Structural and Molecular Biology Lab (Varma Lab)
Advanced Centre for Treatment, Research and Education in Cancer (ACTREC)
Khargar, Navi Mumbai
E-mail: schakrabo...@actrec.gov.in <mailto:schakrabo...@actrec.gov.in>
------------------------------------------------------------------------
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
<https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1>
--
Nicolas Foos PhD - ARISE fellow
https://orcid.org/0000-0003-2331-8399
EMBL Grenoble, McCarthy Team
71 av. des Martyrs,
38000 Grenoble FRANCE
+33 4 57 42 84 67
########################################################################
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list
hosted by www.jiscmail.ac.uk, terms & conditions are available at
https://www.jiscmail.ac.uk/policyandsecurity/
