Hello, are the low resolution data complete? How do the statistics look like (completeness, I/sigma etc.)? Missing low resolution data (e.g. hidden by the beam stop) can create problems in MR. Any axis aligned along the rotation axis by bad luck (would benefit from offset during data collection)? Ice rings or other problems leading to rejections in a systematic region?
Best, Bruno From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> On Behalf Of Shubhashish Chakraborty Sent: 03 March 2022 05:44 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] MR solution not working Hello, I am trying to solve a dataset using molecular replacement. However, neither Phaser MR nor Molrep can give any solution. In Phaser, I have received an advisory that Top FTF has not packed. I have tried molecular replacement using the wild-type protein at different resolutions (I am working on a mutant). Also, I have truncated the loops from the input structure. However, none have worked. So, what can be the possible way to solve this data set? Thank you Shubhashish Chakraborty PhD JRF 2018 Structural and Molecular Biology Lab (Varma Lab) Advanced Centre for Treatment, Research and Education in Cancer (ACTREC) Khargar, Navi Mumbai E-mail: schakrabo...@actrec.gov.in<mailto:schakrabo...@actrec.gov.in> ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
