On Wednesday, 2 March 2022 20:43:42 PST Shubhashish Chakraborty wrote:
> Hello,
> I am trying to solve a dataset using molecular replacement. However, neither
> Phaser MR nor Molrep can give any solution.
> In Phaser, I have received an advisory that Top FTF has not packed.
> I have tried molecular replacement using the wild-type protein at different
> resolutions (I am working on a mutant).
> Also, I have truncated the loops from the input structure. However, none have
> worked.
> So, what can be the possible way to solve this data set?
Double check your space group.
Then check it again.
If there is any chance you integrated the data in the wrong space
group, go back and process it again with lower symmetry.
Break the model into domains; feed phaser the individual domains for
separate placement.
good luck
Ethan
>
> Thank you
>
> Shubhashish Chakraborty
> PhD JRF 2018
> Structural and Molecular Biology Lab (Varma Lab)
> Advanced Centre for Treatment, Research and Education in Cancer (ACTREC)
> Khargar, Navi Mumbai
> E-mail: schakrabo...@actrec.gov.in
>
>
>
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--
Ethan A Merritt
Biomolecular Structure Center, K-428 Health Sciences Bldg
MS 357742, University of Washington, Seattle 98195-7742
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