*Dear Saif* *Hope you are doing well and safe!*
1) How much change in Tm (ΔTm) in a thermal shift assay is considered to be significant ? *As it has already been mentioned there is no specific cutoff for deltaTm to be considered significant. DeltaTm depends on many factors, including the type of dye you use, protein structure and where your compound binds*. 2) A negative ΔTm infers that the compound is making the protein unstable. In such a case, will the co-crystallization be difficult or just impossible or on the contrary it shouldn't matter much? *A negative detaTm means the compound is binding to a different conformational state of the protein, compared to the native one. I would not consider co-crystallization more difficult or impossible in the presence of those compounds, but I would definitely screen for different crystallization conditions.* *Good luck* *Cristy* *#womeninscience* Em qui., 25 de fev. de 2021 às 11:56, Saif Mohd <[email protected]> escreveu: > Hello everyone, > > 1) How much change in Tm (ΔTm) in a thermal shift assay is considered to > be significant ? > > 2) A negative ΔTm infers that the compound is making the protein > unstable. In such a case, will the co-crystallization be difficult or just > impossible or on the contrary it shouldn't matter much? > > > Thanks and best regards, > Saif > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
