Hello Anamika, >From the information you gave, you have two different promoters- araC and the >lac promoter. LacI is the lac inhibitor. The lac promoter is a two part >system- when lactose is not present, the inhibitor sits near the promoter and >blocks transcription of genes downstream. Almost any E. coli system will work the expression of proteins from these promoters, excepting those that have been modified in some way to block arabinose or lactose use. The BL21 (DE3) system has the T7 system implemented, but as you are not using the T7 promoter, there is no particular reason to use this version of E. coli. Best of luck, tom
Tom Peat, PhD Proteins Group Biomedical Program, CSIRO 343 Royal Parade Parkville, VIC, 3052 +613 9662 7304 +614 57 539 419 [email protected] ________________________________ From: CCP4 bulletin board <[email protected]> on behalf of Anamika Singh <[email protected]> Sent: Thursday, November 26, 2020 8:59 PM To: [email protected] <[email protected]> Subject: [ccp4bb] OFF TOPIC question Hi all, I have two constructs having different ori, p15ori and M13 ori, different promoters araC and LacI, and different antibiotic resistance chloramphenicol and Ampicillin respectively. I would like to know which expressing E. coli host cells will be good for the co-transformation of these constructs? Thanks Anamika ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
