BL21(DE3) pLysS using 5X KCM and heat shock transformation worked for me. Crissy L Tarver Postdoctoral Researcher Department of Structural Biology Stanford University School of Medicine ________________________________ From: CCP4 bulletin board <[email protected]> on behalf of Hughes, Jonathan <[email protected]> Sent: Thursday, November 26, 2020 2:48:24 AM To: [email protected] <[email protected]> Subject: [ccp4bb] AW: [ccp4bb] OFF TOPIC question
Bl21Pro j Von: CCP4 bulletin board <[email protected]> Im Auftrag von Anamika Singh Gesendet: Donnerstag, 26. November 2020 11:07 An: [email protected] Betreff: Re: [ccp4bb] OFF TOPIC question One correction to the previous question: I have two constructs having different ori, p15ori and M13 ori, different promoters araBAD promoter and LacI, and different antibiotic resistance chloramphenicol and Ampicillin respectively. I would like to know which E. coli host cells will be good for the co-transformation of these constructs? On Thu, 26 Nov 2020 at 11:59, Anamika Singh <[email protected]<mailto:[email protected]>> wrote: Hi all, I have two constructs having different ori, p15ori and M13 ori, different promoters araC and LacI, and different antibiotic resistance chloramphenicol and Ampicillin respectively. I would like to know which expressing E. coli host cells will be good for the co-transformation of these constructs? Thanks Anamika -- Dr. Anamika Singh Post-Doctoral Fellow Silberman Institute of Life Sciences Hebrew University of Jerusalem, Israel No: 054-294-8036 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
