Hi Group: I have a peptide that got crystallized within 20-25 minutes after putting down the tray. The condition has 1.8M Ammonium Salt, 0.01 Cobalt (ii) Chloride hexahydrate and MES (0.1M) pH 6.5. I dissolved my peptide in 25 mM Sodium Accetate (pH=5). PI of my peptide is 10.2 When I checked for the first time, to my surprise, I saw crystals in the above mentioned condition. After an hour upon checking the same drops, I was unable to find the crystals and I think they are dissolved.
My questions are following: 1) Can we stop the crystals getting dissolved? I have put another tray at 4 degree C, hoping that the crystals (if they appear) will be more stable. 2) Can I use the drop (in which I got crystals) for seeding? Thank You Prasun ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
