Phil Jefferey was right about the point group (432), which looks like it represents about 0.7 % of the PDB! I tested the completeness with MOSFLM strategy for various sporadic missetting angles and 11 degrees of data does give you around 90 % completeness or more with redundancy close to 2, assuming no detector offsets.
Jon Cooper
On 23 Jun 2020 16:10, bogba...@yahoo.co.uk wrote:
Someone told me there is a cubic space group where you can get away with something like 11 degrees of data. It would be interesting if that's correct. These minimum ranges for data collection rely on the crystal being pre-oriented, which is unheard-of these days, although they can help if someone is nagging you to get off the beam line or if your diffraction fades quickly. Going for 180 degrees always makes sense for a well-behaved crystal, or 360 degrees if you want super anomalous differences. Hope this helps a bit.Jon CooperOn 23 Jun 2020 07:29, Andreas Förster <andreas.foerster@DECTRIS.COM> wrote:Hi Murpholino,in my opinion (*), the question is neither number of frames nor degrees. The only thing that matters to your crystal is dose. How many photons does your crystal take before it dies? Consequently, the question to ask is How best to use photons. Some people have done exactly that.
All best.Andreas(*) Disclaimer: I benefit when you use PILATUS or EIGER - but I want you to use them to your advantage.On Tue, Jun 23, 2020 at 12:04 AM Murpholino Peligro <murpholinox@gmail.com> wrote:Hi.
Quick question...I have seen *somewhere* that to get a 'full dataset we need to collect n frames':at least 180 frames if symmetry is Xat least 90 frames if symmetry is Yat least 45 frames if symmetry is ZCan somebody point where is *somewhere*?...also...what other factors can change n... besides symmetry and radiation damage?Thanks
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