Dear Klemens, I am going to setup the crystallisation of the entire protein anyhow. I hope I get lucky :)
Thanks Chitra On Thu, Mar 12, 2020 at 5:12 PM Klemens Wild < klemens.w...@bzh.uni-heidelberg.de> wrote: > On 12.03.20 08:53, chitra latka wrote: > > Dear All, > > I am working on a protein that has flexible C terminus. None of the > available structures even in homologs have density for C term region > (around 20 odd residues). All the available pdb entries have missing > density for these 20 residues at C terminus. > > I am going to try my luck crystallising the entire protein in hope of > getting density for C term residues as well (Fingers crossed). > > Has anyone faced a similar problem where they have managed to get density > for a flexible terminus successfully? > > Any suggestions would be appreciated. > > Cheers ! > > Chitra Latka > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > Dear Chitra > > I would nevertheless try. Sometimes flexible termini fold back either in > cis or in trans (crystal packing, a case I just had Yesterday) and you > might learn sth important for biological regulation if you are lucky. At > the same time I would truncate the terminus and crystallize the globular > domain in parallel. > > Good luck > > Klemens > -- Regards Chitra ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
