Dear Chitra, There usually is a reason the C-terminus is disordered. Either it needs to bind a ligand to get ordered, or it needs to bind to some other protein. You have to check the literature. If the C-terminus binds a ligand, you have to add this ligand to your crystallization experiment. If it binds to some other protein, you could try cocrystallization of both proteins, or just try to cocrystallize the other protein with only the 20 residues or so of the C-terminus.
Best, Herman Von: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> Im Auftrag von chitra latka Gesendet: Donnerstag, 12. März 2020 08:54 An: CCP4BB@JISCMAIL.AC.UK Betreff: [EXTERNAL] [ccp4bb] Flexible C terminus EXTERNAL : Real sender is owner-ccp...@jiscmail.ac.uk<mailto:owner-ccp...@jiscmail.ac.uk> Dear All, I am working on a protein that has flexible C terminus. None of the available structures even in homologs have density for C term region (around 20 odd residues). All the available pdb entries have missing density for these 20 residues at C terminus. I am going to try my luck crystallising the entire protein in hope of getting density for C term residues as well (Fingers crossed). Has anyone faced a similar problem where they have managed to get density for a flexible terminus successfully? Any suggestions would be appreciated. Cheers ! Chitra Latka ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=tHXRPIRekvSgMSDPEVP15ta2iH1g8NkqcZVMEpk1VLo&s=k0Yuiu6SCu5YkIBlnejKmh7Eq9F_cX3TLtdqQZVyR3w&e=> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
