Dear Herman, Its a two domain protein and the second domain gets chopped off and stabilises the other domain by binding near the flexible C - terminus of first domain. I am trying to crystallise both the proteins together. Literature review doesn't report binding of the protein to any other protein. So, it kind of has the ligand or another domain to stabilise but eve then density of C-terminus residues remain missing.
Thanks Chitra Latka On Thu, Mar 12, 2020 at 4:08 PM Schreuder, Herman /DE < herman.schreu...@sanofi.com> wrote: > Dear Chitra, > > > > There usually is a reason the C-terminus is disordered. Either it needs to > bind a ligand to get ordered, or it needs to bind to some other protein. > You have to check the literature. If the C-terminus binds a ligand, you > have to add this ligand to your crystallization experiment. If it binds to > some other protein, you could try cocrystallization of both proteins, or > just try to cocrystallize the other protein with only the 20 residues or so > of the C-terminus. > > > > Best, Herman > > > > *Von:* CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> *Im Auftrag von *chitra > latka > *Gesendet:* Donnerstag, 12. März 2020 08:54 > *An:* CCP4BB@JISCMAIL.AC.UK > *Betreff:* [EXTERNAL] [ccp4bb] Flexible C terminus > > > > *EXTERNAL : *Real sender is owner-ccp...@jiscmail.ac.uk > > > > Dear All, > > > > I am working on a protein that has flexible C terminus. None of the > available structures even in homologs have density for C term region > (around 20 odd residues). All the available pdb entries have missing > density for these 20 residues at C terminus. > > > > I am going to try my luck crystallising the entire protein in hope of > getting density for C term residues as well (Fingers crossed). > > > > Has anyone faced a similar problem where they have managed to get density > for a flexible terminus successfully? > > > > Any suggestions would be appreciated. > > > > Cheers ! > > > > Chitra Latka > > > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=tHXRPIRekvSgMSDPEVP15ta2iH1g8NkqcZVMEpk1VLo&s=k0Yuiu6SCu5YkIBlnejKmh7Eq9F_cX3TLtdqQZVyR3w&e=> > -- Regards Chitra ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
