Something that pops up here routinely, connected to 5) what does "resolution" really mean?:
While new methods are being implemented to improve the accuracy of models or to deal with weak data, how to convince editors to accept the new methods? cheers, matthias Dr. Matthias Barone AG Kuehne, Rational Drug Design Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP) Robert-Rössle-Strasse 10 13125 Berlin Germany Phone: +49 (0)30 94793-284 ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Frank Von Delft <frank.vonde...@sgc.ox.ac.uk> Sent: Tuesday, July 16, 2019 6:34:16 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] challenges in structural biology 1.1 Getting many diverse conformations routinely into well-diffracting crystals; and knowing how to interpret them biologically. On 15/07/2019 20:44, Holton, James M wrote: > Hello folks, > > I have the distinct honor of chairing the next Gordon Research > Conference on Diffraction Methods in Structural Biology (July 26-31 > 2020). This meeting will focus on the biggest challenges currently > faced by structural biologists, and I mean actual real-world > challenges. As much as possible, these challenges will take the form of > friendly competitions with defined parameters, data, a scoring system, > and "winners", to be established along with other unpublished results > only at the meeting, as is tradition at GRCs. > > But what are the principle challenges in biological structure > determination today? I of course have my own ideas, but I feel like I'm > forgetting something. Obvious choices are: > 1) getting crystals to diffract better > 2) building models into low-resolution maps (after failing at #1) > 3) telling if a ligand is really there or not > 4) the phase problem (dealing with weak signal, twinning and > pseudotranslation) > 5) what does "resolution" really mean? > 6) why are macromolecular R factors so much higher than small-molecule ones? > 7) what is the best way to process serial crystallography data? > 8) how should one deal with non-isomorphism in multi-crystal methods? > 9) what is the "structure" of something that won't sit still? > > What am I missing? Is industry facing different problems than > academics? Are there specific challenges facing electron-based > techniques? If so, could the combined strength of all the world's > methods developers solve them? I'm interested in hearing the voice of > this community. On or off-list is fine. > > -James Holton > MAD Scientist > > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
